BMMs seeded onto bovine bone slices placed in a 96-well plate at a density of 1×104 cells/well were cultured in complete α-MEM containing 25 ng/mL M-CSF and 100 ng/mL RANKL for 4 days until osteoclasts began to form. The cells were further treated with different concentrations of Pri (0, 25, 50, or 100 nM) for 10 days. The slides were washed with 6% sodium hypochlorite solution to remove the cells and resorption pits were imaged using scanning electron microscopy (Thermo Fisher Scientific). The percentage of resorption area relative to total area under each treatment condition were measured using Image J software.
Scanning electron microscopy
Manufactured by Thermo Fisher Scientific
Sourced in United States
Scanning electron microscopy is an analytical technique that uses a focused beam of high-energy electrons to generate a variety of signals from the surface of a sample. The signals provide information about the sample's surface topography, composition, and other properties.
Automatically generated - may contain errors
Lab products found in correlation
Auto fine coater, by JEOL (1 mentions)
Hepes buffer, by Merck Group (1 mentions)
K850 critical point drier cpd, by Quorum Technologies (1 mentions)
Sc7620 mini sputter coater, by Quorum Technologies (1 mentions)
Quanta 250 feg sem, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Thincert well inserts, by Greiner (1 mentions)
Nova nanosem 630, by Thermo Fisher Scientific (1 mentions)
Irgacure 651, by Novartis (1 mentions)
Centrifuge, by Eppendorf (1 mentions)
14 protocols using scanning electron microscopy
1
Osteoclast Differentiation and Resorption Assay
2
Preparation and Characterization of Amyloid Fibrils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh Aβ1–40 solution was prepared as previously described (Watts et al., 2010; Li et al., 2011). Aβ1–40 peptides (AnaSpec, Fremont, CA, USA) were diluted with sterile 0.1 M PBS (pH 7.4; Affymetrix USB, Santa Clara, CA, USA). The solution was sonicated for 1 minute and incubated at 37°C for 1 week to aggregate the peptides. This stock solution of Aβ1–40 was stored at –20°C (Resende et al., 2008).
scanning electron microscopy (SEM) images of amyloid fibrils were obtained using an ESEM system (Thermo Fisher Scientific, Waltham, MA, USA) with an accelerating voltage of 5 kV. The sample solution (1 nmol/µL) was diluted 50-fold in pure water, and 1 µL of the diluted solution was placed on silicone and air-dried overnight. To prevent electric charge build-up, the sample was coated with platinum using an Auto-Fine Coater (JEOL, Akishima, Tokyo, Japan) prior to imaging. Images were viewed at magnifications of 2000×, 4000×, and 10,000×. Fibril’s diameter data were collected from software build-up in scanning electron microscopy (Thermo Fisher Scientific, Waltham, MA, USA).
scanning electron microscopy (SEM) images of amyloid fibrils were obtained using an ESEM system (Thermo Fisher Scientific, Waltham, MA, USA) with an accelerating voltage of 5 kV. The sample solution (1 nmol/µL) was diluted 50-fold in pure water, and 1 µL of the diluted solution was placed on silicone and air-dried overnight. To prevent electric charge build-up, the sample was coated with platinum using an Auto-Fine Coater (JEOL, Akishima, Tokyo, Japan) prior to imaging. Images were viewed at magnifications of 2000×, 4000×, and 10,000×. Fibril’s diameter data were collected from software build-up in scanning electron microscopy (Thermo Fisher Scientific, Waltham, MA, USA).
3
SEM Analysis of Sample Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SEM (Scanning Electron Microscopy, Thermo Fisher Scientific, Eindhoven, The Netherlands) analysis of the samples was performed using the Phenom Pure Pro X electronic microscope (Thermo Fisher Scientific, Eindhoven, The Netherlands) and Phenom Pro Suite acquisition software (2015, Thermo Fisher Scientific, Eindhoven, The Netherlands). This device integrates an optical microscope with 20× optical zoom and an electronic microscope with a magnification range of 70–30,000×.
Samples were prepared according to the following protocol: Impurities were removed from the samples’ surface using a compressed air can, then a conductive double-sided tape was glued to a supportive device (which is cylindrical, with a diameter of 10 mm). After each sample was glued to the device, it was inserted in a charge reduction sample holder, which will be placed in the microscope. This equipment has the following advantage: for a non-metallic sample, it reduces the electron charge; therefore, the preparation of nonconductive samples is no longer necessary. After the device with the sample is inserted, the image of the sample is displayed, and the areas of the SEM are defined. For this analysis, the microscope settings were the following: intensity of electron cannon 10 keV, magnification 320x, and working distance 3 mm under the cylindrical device.
Samples were prepared according to the following protocol: Impurities were removed from the samples’ surface using a compressed air can, then a conductive double-sided tape was glued to a supportive device (which is cylindrical, with a diameter of 10 mm). After each sample was glued to the device, it was inserted in a charge reduction sample holder, which will be placed in the microscope. This equipment has the following advantage: for a non-metallic sample, it reduces the electron charge; therefore, the preparation of nonconductive samples is no longer necessary. After the device with the sample is inserted, the image of the sample is displayed, and the areas of the SEM are defined. For this analysis, the microscope settings were the following: intensity of electron cannon 10 keV, magnification 320x, and working distance 3 mm under the cylindrical device.
4
Characterizing Fmoc-FF/S Hydrogel Morphology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The morphologies of Fmoc-FF/S hydrogels with and without incorporated collagens were analysed by Scanning Electron Microscopy (SEM, Thermo Fisher Scientific, Loughborough, UK). Briefly, hydrogels were prepared by pipetting ~300 μL of the pre-gel solutions into Thin-Cert well inserts (0.4 μm pore size Greiner Bio-One Ltd., Stonehouse, UK). The inserts were then placed into 24-well plates and incubated at 37 °C with a total volume of 1.3 mL PBS containing the protein of interest to fully crosslink the hydrogels. After 24 h, hydrogels were fixed in 2.5% (w/v) glutaraldehyde (Sigma-Aldrich, Welwyn Garden City, UK) and 4% (w/v) paraformaldehyde (Sigma-Aldrich, Welwyn Garden City, UK) in 0.1 M HEPES buffer (Sigma-Aldrich, Welwyn Garden City, UK). After rinsing the samples in PBS, all samples were dehydrated in a graded ethanol (EtOH) series (25, 50, 75, 95, and 100% v/v EtOH/water). Samples were maintained at 100% EtOH and dried in a K850 Critical Point Drier (CPD, Quorum Technologies, Lewes, UK). After the CPD step, samples were transferred into metallic pins and coated with gold palladium alloy using an SC7620 Mini Sputter Coater (Quorum, Lewes, UK). Samples were then imaged on a Quanta 250 FEG SEM (Thermo Fisher Scientific, Loughborough, UK) at 20 kV.
5
Structural Analysis of ZnO Polymer Nanocomposites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
X-ray diffraction (XRD) (Rigaku Ultra high-resolution triple axis multiple reflection SmartLab X-ray Diffraction System, Japan) and Scanning Electron Microscopy (SEM) (FEI Company, Hillsborough, OR, USA) were used to analyze the structuring, the morphology and the distribution of ZnO within the polymer nanocomposites and microcomposites. The structural analysis was performed by the XRD method using a Rigaku Ultra high-resolution triple axis multiple reflection SmartLab X-ray Diffraction System in wide-angle measurements mode—WAXRD. The structural features (e.g., crystallinity and lattice constant) were investigated using a 9 kW rotating anode X-ray diffraction system that employs Cu Kα1 radiation (λ = 1.5406 Å). WA-XRD (Wide Angle X-Ray Diffraction) and patterns were recorded in θ/2θ scan at 5°/min from 10 to 60°. Each sample was mounted on an aluminum holder.
Detailed SEM characterization was performed using a (FE-SEM) Nova NanoSEM 630 (FEI Company, Hillsborough, OR, USA), equipped with an EDX detector (EDAX TEAM™, EDAX Inc., Mahwah, NJ, USA) and field emission microscope. All samples were characterized in high vacuum mode. In order to correctly perform the SEM characterization, samples were coated by thermal evaporation with a 5 nm Au thin film to avoid charging during the analysis.
Detailed SEM characterization was performed using a (FE-SEM) Nova NanoSEM 630 (FEI Company, Hillsborough, OR, USA), equipped with an EDX detector (EDAX TEAM™, EDAX Inc., Mahwah, NJ, USA) and field emission microscope. All samples were characterized in high vacuum mode. In order to correctly perform the SEM characterization, samples were coated by thermal evaporation with a 5 nm Au thin film to avoid charging during the analysis.
6
In Situ Electrochemical Sensor Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Due to bulky shape of MIPs1 obtained using the first approach and an insufficient reaction yield of the second approach (MIPs2) an in situ MIPs polymerization, directly on the electrode surface was investigated. In situ polymerization was prepared with the same pre-polymerization mixture as used in the second approach, implementing two kinds of initiators, AIBN and Ciba® IRGACURE® 651 in order to choose the best method. Thermal polymerization (MIPs3) was initiated by addition of 2,2-azobis-2-isobutyronitrile (AIBN), and the reaction was carried out for 24 h under 60 °C. UV polymerization (MIPs4) was initiated by Ciba® IRGACURE® 651 (2,2-Dimethoxy-1,2-diphenylethan-1-one), and performed under UV lamps with UV wave length range between 300-400 nm, for 1 hour.
The morphologies of microspherical MIPs particles prepared by bulk, precipitation and in situ polymerization were compared using Scanning Electron Microscopy (SEM, FEI Company, Eindhoven
The Netherlands, (Fig. 1). For reference, these MIPs were compared with commercial MIPs towards amphetamine (Fig. 1d). The obtained functionalized electrodes were eventually tested using an electrochemical detection of N-FA performed using an automated flow injection system, developed by CapSenze AB (Lund, Sweden).
The morphologies of microspherical MIPs particles prepared by bulk, precipitation and in situ polymerization were compared using Scanning Electron Microscopy (SEM, FEI Company, Eindhoven
The Netherlands, (Fig. 1). For reference, these MIPs were compared with commercial MIPs towards amphetamine (Fig. 1d). The obtained functionalized electrodes were eventually tested using an electrochemical detection of N-FA performed using an automated flow injection system, developed by CapSenze AB (Lund, Sweden).
7
Bacterial Cell Morphology Observation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to the previously reported method [30 (link),31 (link),32 (link)], the changes in bacterial cell morphology were observed via scanning electron microscopy (FEI, Hillsboro, OR, USA). The details of the assay were adequately described in the published previous paper.
8
Scanning Electron Microscopy of Plant Shoot Apex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Shoot apex tissue from the vegetative apices of WT and paa1019 plants was collected and dissected carefully under a sterol microscope, and subsequently fixed in 2.5% glutaraldehyde in 0.1 M phosphate-buffered saline (PBS; 4 mM sodium phosphate, 200 mM NaCl, pH 7.2) at 4°C overnight. After rinsing twice with PBS, the samples were rinsed in 4% (w/v) sucrose solution for 5 min, then dehydrated using a graded series of ethanol (30, 50, 70, 80, 90, 95, and 100%). Finally, the samples were dried in a critical-point drier, sputter-coated with platinum, and observed under bright-field by scanning electron microscopy (Inspect, FEI, United States).
9
Scanning Electron Microscopy of Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scanning electron microscopy (FEI, Hillsboro, OR, USA) was used to analyze the surface morphology of VCZ-MA-NPs and VCZ-MP-NPs. Images were recorded using a scanning electron microscope with an Everhart-Thornley detector under a vacuum. The samples were posed on carbon adhesive tape to hold the samples on the stub, followed by gold coating for three consecutive cycles. The images were recorded at a voltage of 10.00 kV [42 (link)].
10
Characterizing GNEC/HAPAAm Hydrogel Microstructure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The internal microstructures of the GNEC/HAPAAm hydrogels were observed by scanning electron microscopy (FEI Scios). Before observation, the hydrogel samples were broken in liquid nitrogen, freeze-dried, and finally observed by SEM.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!