MOLM-13 (DMSZ # ACC554), F36-P (DMSZ # ACC543) and SKK-1 have been obtained from DSMZ as a collaboration with Hans Drexler and have been characterized in detail36 (link). SKK-1 cells are available from the original authors37 (link). MDS-L cells were kindly provided by Tohyama and colleagues61 (link).
Cells were maintained in RPMI 1640 (Gibco, Thermo Scientific, Waltham, MA) supplemented with 10% of fetal bovine serum (FBS), 1% penicillin–streptomycin and 1%l -Gutamine (Gibco) at 37 °C in 5% CO2. HEK293T (ATCC #CRL-11268) and HS-5 (ATCC #CRL-11882) were obtained from American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s media (Gibco) supplemented with 10% FBS, 1% penicillin–streptomycin and 1% l -Glutamine (Gibco) at 37 °C in 5% CO2. Cells were authenticated and passaged for <6 months. CD34+ cells were isolated from mononuclear cells of healthy donors (see Supplementary Data 2 for donor characteristics) and cultured in suspension as described62 (link). For combinatorial treatments, cells were treated with AZA (Sigma-Aldrich, St. Louis, MO), decitabine (Sigma-Aldrich), C646 (Sigma-Aldrich), A-485 (Tocris, Bristol, UK), iCBP-112 (Tocris), CCS1477 (CellCentric, Ltd, Cambridge, UK), JQ-1 (Sigma-Aldrich), DMSO (Sigma-Aldrich) or CHX (Sigma-Aldrich) at the indicated amounts.
Cells were maintained in RPMI 1640 (Gibco, Thermo Scientific, Waltham, MA) supplemented with 10% of fetal bovine serum (FBS), 1% penicillin–streptomycin and 1%