The sample was taken from a 34-year-old male who died from a road traffic accident. Gingival tissue sample was taken at a PMI of 2 h and 4 h and immediately fixed in 2.5% buffered glutaraldehyde overnight at 4°C followed by fixing with osmium tetroxide. Tissue bits were subjected to dehydration in ascending grades of acetone and then embedded using Epon 812 resin mixture. The tissues were sectioned using Leica Ultracut R Ultramicrotome with diamond knives. The ultra-thin sections were stained with uranyl acetate and lead acetate. These sections were screened in JEOL JEM 1400 Transmission Electron Microscope at 80 kV. The micrographs were taken using the Olympus Keen view CCD camera attached to the microscope.
Jem 1400 transmission electron microscope
Manufactured by Olympus
Sourced in Japan
The JEM 1400 Transmission Electron Microscope is a high-performance instrument designed for advanced material analysis. It utilizes a beam of electrons to generate detailed images and diffraction patterns of samples at the nanoscale level. The JEM 1400 provides high-resolution imaging capabilities and supports a range of analytical techniques for studying the microstructure and composition of various materials.
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Lab products found in correlation
3 protocols using jem 1400 transmission electron microscope
1
Ultrastructural Analysis of Gingival Tissues
2
Negative Staining of Protein Fibrils
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A Pelco Easi-glo discharge unit was used to glow discharge a carbon-coated mesh copper grid. Afterward, 5 μl of the sample (fibril or oligomer) was incubated on the grid for 1 min. The grid was washed with water three times, washed with stain (uranyl acetate 1% w/v) twice, incubated with stain for 30 s, washed with water, and blotted with filter paper to remove excess liquid from the grid. The grid was allowed to dry before proceeding with EM imaging using a JEOL JEM 1400 transmission electron microscope operated at 100 kV and equipped with an Olympus-SIS Veleta side-mount 2K × 2K digital camera.
3
Ultrastructural Analysis of Skeletal Muscle
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After being cut into sections with 1-mm thicknesses, fresh quadriceps muscle tissue was fixed in 2.5% glutaraldehyde for 2 h followed by 1% osmium tetroxide. Sections were then stained with uranyl acetate, dehydrated in a methanol series and propylene oxide, and embedded with Epon-812. Ultrathin sections were obtained, and ultrastructural changes and mitophagy of skeletal muscle cells were examined using a JEM-1400 transmission electron microscope (OLYMPUS, Japan).
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