Mouse rat total insulin high sensitivity assay kit

At 12 weeks of age, adult male mice were fasted for 12 h and then dissected under isoflurane inhalation anesthesia (5% induction, 2% maintenance). Blood was collected from the heart by cardiac puncture via a midline incision. Blood glucose levels were measured using a Stat Strip XP2 (Nipro, Osaka, Japan). Next, serum was separated from total blood by centrifugation at 3000 rpm for 5 min and stored at −20 °C. Serum insulin was assessed for immunoreactive insulin levels (IRI) using a mouse/rat total insulin (high sensitivity) assay kit (Immuno-Biological Laboratories Co., Fujioka, Gunma, Japan). Serum was also assayed for insulin resistance using the human formula for the homeostasis model assessment of insulin resistance (HOMA-IR) [19 (link)].

Blood glucose levels after 12 h of fasting were measured using a Stat Strip XP2 (Nipro Corp., Osaka, Japan). Blood was then centrifuged at 3000 rpm for 5 min at room temperature and the serum was stored at −20 °C. Serum IRI levels were measured using a mouse/rat total insulin (high sensitivity) assay kit (Immuno-Biological Laboratories Co., Ltd., Fujioka, Gunma, Japan). Homeostasis model assessment of insulin resistance (HOMA-R) was calculated using the human formula:
since there is no formula for mice [15 (link)].