Camk2bA303R/A303Rand Camk2bT287A/T287A mice were tested in a 129P2-C57Bl/6OlaHsd F2 hybrid background. Camk2bΔex2/Δex2mice were backcrossed >16 times into C57Bl/6JOlaHsd background. All conditional mice used in this study were backcrossed 10–12 times into the C57BL/6JOlaHsd background and crossed with cre lines maintained in the C57BL/6JOlaHsd background. Mice were genotyped when they were 7–10 days old, and re-genotyped after the mice were sacrificed. Genotyping records were obtained and kept by a technician not involved in the experimental design, performance and analysis. All mice were tested between 2–3 months of age, except the Camk2bf/f;Rgs9-cre group, which was tested between 3–5 months of age. All mice were kept group-housed in IVC cages (Sealsafe 1145 T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21 °C (±1 °C), humidity at 40–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. For all experiments mutants were compared to WT or cre-negative homozygous floxed littermates. All groups were matched for age and sex and all experiments were done during daytime. Experimenters were blind for genotype throughout experiments and data analysis. All research was performed in accordance with and approved by a Dutch Animal Ethical Committee (DEC) for animal research.
Sealsafe 1145t
Manufactured by Tecniplast
Sourced in Italy
The Sealsafe 1145T is a lab equipment product designed for animal housing. It provides a controlled environment for housing laboratory animals. The core function of the Sealsafe 1145T is to maintain temperature, humidity, and air circulation within a defined space.
Automatically generated - may contain errors
8 protocols using sealsafe 1145t
1
Transgenic Mice for CaMKII Research
2
FvB/NHsD and C57Bl6/J Mouse Breeding
3
Neuronal Cultures from FvB/NHsD Mice
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FvB/NHsD females were crossed with FvB/NHsD males (ordered at 8–10 weeks old from Envigo) for the neuronal cultures. Mice were kept group-housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21 ∘C(±1)∘C, humidity at 40-70%. Food pellets (801727CRM(P) from Special Dietary Service) and water were available ad libitum. All animal experiments were conducted in accordance with the European Commission Council Directive 2010/63/EU (CCD approval AVD101002017893).
4
Acclimating C57BL/6J Mice for Experiments
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Six-week-old C57BL/6J mice (16 male and 16 female mice) were obtained from Charles River Laboratories (Maastricht, the Netherlands). Upon arrival, the mice were housed for 1 week in groups of three animals in standard laboratory cages (Sealsafe 1145T, Tecniplast, Buguggiate, Italy; 36.9 cm L × 15.6 cm W × 13.2 cm H) with bedding material (Lignocel BK 8/15, J. Rettenmaier & Söhne GmbH, Rosenberg, Germany) and nesting material (facial tissue paper, Tork, SCA Hygiene Products, Zeist, the Netherlands) on a 12:12-h light-dark cycle (lights on at 8 a.m.), at temperatures of 21–23 °C with 40–70% relative humidity. Chow food pellets (801727 CRM (P), Special Diets Services, Essex, UK) and water were available ad libitum. After the 1-week acclimatization, the mice were housed individually in a cage of the same setting for 3 days before the first series of TPTs was started. Thus, the mice were about 8 weeks old when enrolled in the experimental setups. All experimental procedures were performed with the approval of the Animal Ethics Committee at Erasmus MC, Rotterdam, the Netherlands.
5
Murine Neuronal Culture and In Utero Electroporation
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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8–10 weeks old from Envigo). For the in utero electroporation, female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group‐housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21°C (±1°C), humidity at 40–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were approved by the Local Animal Experimentation Ethical Committee, in accordance with Institutional Animal Care and Use Committee guidelines.
6
Mouse Breeding and Housing for Neuronal Cultures
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FvB/NHsD females were crossed with FvB/NHsD males (ordered at 8–10 weeks old from Envigo) for the neuronal cultures, whereas for the in utero electroporation female, FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group-housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21 °C (±1 °C), humidity at 40–70%. Food pellets (801727CRM(P) from Special Dietary Service) and water were available ad libitum. All animal experiments were conducted in accordance with the European Commission Council Directive 2010/63/EU (CCD approval AVD101002017893).
7
Murine Neuronal Culture and In Utero Electroporation
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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8–10 weeks old from Envigo). For the in utero electroporation female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group-housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21 °C (±1 °C), humidity at 40–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were approved by the Erasmus MC institutional Animal Care and Ethical Committee, in accordance with European and Institutional Animal Care and Use Committee guidelines.
8
Neuronal Culture Generation and In Utero Electroporation
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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8‐ to 10‐weeks old from Envigo). For the in utero electroporation, female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8‐to 10‐weeks old from Charles River). All mice were kept group‐housed in IVC cages (Sealsafe 1145T; Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12‐h light/dark cycle at 21°C ± 1°C, humidity at 40%–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were conducted in accordance with the European Commission Council Directive 2010/63/EU (CCD approval AVD101002017893).
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