Ptgs2 sirna

NC siRNA (5′-UUCUCCGAACGUGUCACGU-3′; Genepharma, China), FOXQ1 siRNA (5′-CGCGGACUUUGCACUUUGA-3′; Genepharma, China), PTGS2 siRNA (5′-UCAAGUGUUGCACAUAAUCDTDT-3′; Genepharma, China), FOXQ1 siRNA (5′-CGCGGACUUUGCACUUUGA-3′; Genepharma, China) combining PTGS2 siRNA (5′-UCAAGUGUUGCACAUAAUCDTDT-3′; Genepharma, China), CDK5 siRNA (5′-GUCGAUGACCAGUUGAAGATT-3′; Genepharma, China), and FOXQ1 siRNA (5′-CGCGGACUUUGCACUUUGA-3′; Genepharma, China) combining CDK5 siRNA (5′-GUCGAUGACCAGUUGAAGATT-3′; Genepharma, China) were transfected into two cellular AD models (primary neuron AD model and PC-12 cellular AD model) using Lipofectamine™ LTX Reagent with PLUS™ Reagent (Invitrogen, USA). Then the transfected cells were divided into NC group, Si-FOXQ1 group, Si-PTGS2 group, Si-FOXQ1&Si-PTGS2 group, Si-CDK5 group, and Si-FOXQ1&Si-CDK5 group. At 48 h, the cell apoptosis rate was evaluated by Hoechst/PI (Sigma, USA) in accordance with the method in the previous study (Ma et al., 2019 (link)); neurite outgrowth was observed under ×200 magnification with a microscope (Olympus, Japan), and then the TNF-α, IL-1β, and IL-6 in supernatant were measured by ELISA, and the expressions of FOXQ1, PTGS2, and CDK5 in each group were detected by Western blot.