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Stat3 sirna

Manufactured by Qiagen
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STAT3-siRNA is a laboratory reagent used for gene silencing experiments. It is a synthetic, double-stranded small interfering RNA (siRNA) designed to target and suppress the expression of the STAT3 (signal transducer and activator of transcription 3) gene. The core function of STAT3-siRNA is to facilitate the study of STAT3 gene function and its role in cellular processes.

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Lab products found in correlation

Control sirna, by Qiagen (4 mentions) Rnai fect reagent, by Qiagen (3 mentions) Dnmt1 sirna, by OriGene (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Oligofectamine reagent, by Qiagen (1 mentions)

Spelling variants of this product

SiRNAs (104 citations) Hs_STAT3_7 FlexiTube siRNA (2 citations)

6 protocols using stat3 sirna

1

Targeting IL-6, STAT3, and AUF1 in Cells

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IL-6-siRNA, STAT3-siRNA, and control–siRNA were obtained from QIAGEN, USA. AUF1-siRNA (pSILENCER-AUF15), which targets all four AUF1 isoforms [11 (link)], was a generous gift from Dr. Gorospe. The transfections were carried out using the RNAi Fect reagent (QIAGEN) as recommended by the manufacturer.
Al-Ansari M.M., Al-Saif M., Arafah M., Eldali A.M., Tulbah A., Al-Tweigeri T., Semlali A., Khabar K.S, & Aboussekhra A. (2020). Clinical and functional significance of tumor/stromal ATR expression in breast cancer patients. Breast Cancer Research : BCR, 22, 49.
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2

Transfection of Gene Silencing Targets

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The transfections using DNMT1-siRNA (Origene, SR301244A), STAT3-siRNA (Qiagen, USA), HuR-siRNA (Metabion) and control–siRNA were carried out using the RNAi Fect reagent (Qiagen) as recommended by the manufacturer.
Al-Kharashi L.A., Al-Mohanna F.H., Tulbah A, & Aboussekhra A. (2017). The DNA methyl-transferase protein DNMT1 enhances tumor-promoting properties of breast stromal fibroblasts. Oncotarget, 9(2), 2329-2343.
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3

Knockdown of LncTCF7 and STAT3 via siRNA

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LncTCF7 siRNA, STAT3 siRNA and Control siRNA were purchased from Qiagen, Hilden, Germany. Cells were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Forty-eight hours after transfection, cells were harvested and subjected to qRT-PCR or western blot analyses.
Wu J., Zhang J., Shen B., Yin K., Xu J., Gao W, & Zhang L. (2015). Long noncoding RNA lncTCF7, induced by IL-6/STAT3 transactivation, promotes hepatocellular carcinoma aggressiveness through epithelial-mesenchymal transition. Journal of Experimental & Clinical Cancer Research : CR, 34, 116.
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4

STAT3 and OPG Silencing Protocol

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STAT3 siRNA was obtained from Qiagen, OPG siRNA and control siRNA were obtained from (Santa Cruz, Santa Cruz, CA, USA). The transfections were carried out using the Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA) following the protocol recommended by the manufacturer.
Al-Nasrallah H.K., Al-Ansari M.M, & Aboussekhra A. (2022). Osteoprotegerin (OPG) Upregulation Activates Breast Stromal Fibroblasts and Enhances Their Pro-Carcinogenic Effects through the STAT3/IL-6 Signaling. Cells, 11(21), 3369.
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5

STAT3-siRNA and AUF1-siRNA Transfection

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STAT3-siRNA and control–siRNA were obtained from QIAGEN, USA. AUF1-siRNA (pSILENCER-AUF15), which targets all four AUF1 isoforms [35 (link)], was a generous gift from Dr. Gorospe. The transfections were carried out using the RNAi Fect reagent (QIAGEN) as recommended by the manufacturer.
Hendrayani S.F., Al-Harbi B., Al-Ansari M.M., Silva G, & Aboussekhra A. (2016). The inflammatory/cancer-related IL-6/STAT3/NF-κB positive feedback loop includes AUF1 and maintains the active state of breast myofibroblasts. Oncotarget, 7(27), 41974-41985.
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6

STAT3 Silencing Impacts Cell Invasion

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Small-interfering RNA for human STAT3 (STAT3 siRNA) was obtained from Qiagen (Hilden, Germany). AGS and MKN28 cells were seeded in 6-cm dish (Iwaki, Funabashi, Japan) and maintained for 24 h. Then, the cells were transfected with 25 nM STAT3 siRNA or non-silencing siRNA (as a control) using the Oligofectamine reagent (Qiagen) according to the manufacturer's recommendation (Sekikawa et al, 2010 (link)). After incubation for 48 h, cells were washed with PBS, harvested, and subjected to invasion assay.
Fukui H., Zhang X., Sun C., Hara K., Kikuchi S., Yamasaki T., Kondo T., Tomita T., Oshima T., Watari J., Imura J., Fujimori T., Sasako M, & Miwa H. (2014). IL-22 produced by cancer-associated fibroblasts promotes gastric cancer cell invasion via STAT3 and ERK signaling. British Journal of Cancer, 111(4), 763-771.
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