Cell culture supernatants and serum were analyzed with a Mouse RANKL ELISA Kit (Abcam) and Mouse Osteoprotegerin ELISA Kit (Abcam). All experiments were performed according to the manufacturer's protocols.
Mouse rankl elisa kit
Manufactured by Abcam
Sourced in China, United States, Germany
The Mouse RANKL ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of mouse receptor activator of nuclear factor kappa-B ligand (RANKL) levels in cell culture supernatants, serum, and plasma samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse osteoprotegerin elisa kit, by Abcam (2 mentions)
Quantikine elisa, by R&D Systems (2 mentions)
Mouse sclerostin elisa kit, by Boster Bio (1 mentions)
Testosterone elisa kit, by R&D Systems (1 mentions)
Elisa max deluxe set mouse il 6, by BioLegend (1 mentions)
Microvette, by Sarstedt (1 mentions)
Rat mouse pinp eia, by Immunodiagnostic Systems (1 mentions)
Ratlaps ctx 1 eia, by Immunodiagnostic Systems (1 mentions)
5 protocols using mouse rankl elisa kit
1
Quantifying RANKL and Osteoprotegerin Levels
2
Sclerostin and RANKL ELISA Assay
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The culture medium of MLO-Y4 was collected for ELISA. The protein level of sclerostin and RANKL released into the culture supernatants were measured with mouse Sclerostin ELISA kit (Boster, Wuhan, China) and mouse RANKL ELISA kit (Abcam, USA), according to product’s datasheet.
3
Osteogenesis and Testosterone Markers in Prdx5KO Mice
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The levels of specific markers of osteogenesis in the serum from WT and Prdx5Ko mice at 12 weeks were measured using enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s description. The RANKL levels were measured using a mouse RANKL ELISA kit (Abcam); the OPG levels were measured using a Quantikine ELISA (R&D Systems) kit; and the BMP2 levels were measured using mouse BMP2 ELISA kits, respectively (LSBio).
The testosterone levels in the serum from WT and Prdx5Ko male mice at 4, 8, and 12 weeks were measured using the testosterone ELISA kit (R&D Systems) according to the manufacturer’s description.
The testosterone levels in the serum from WT and Prdx5Ko male mice at 4, 8, and 12 weeks were measured using the testosterone ELISA kit (R&D Systems) according to the manufacturer’s description.
4
Serum Biomarkers in Ovariectomized Mice
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The serum was collected from the SHAM, OVX, and OVX + NAPMA mice before euthanasia after five weeks of NAPMA treatment. Serum CTX-1 levels were measured using a RatLaps EIA kit (IDS Nordic, Herlev, Denmark). Serum RANKL levels were measured using a Mouse RANKL ELISA kit (Abcam, Cambridge, MA, USA). Serum OPG levels were measured using a Quantikine ELISA (R&D system, Minneapolis, MN, USA). Serum interleukin-6 levels were measured using an ELISA MAX™ Deluxe Set Mouse IL-6 (BioLegend, San Diego, CA, USA).
5
Bone Turnover Markers and Cytokines in Ovariectomized Mice
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To determine the concentrations of bone turnover markers (procollagen type I N-terminal propeptide (PINP); OPG; C-terminal telopeptide (CTX); RANKL), blood was harvested at an age of 12 or 36 weeks and 8 weeks after OVX or sham operation in microvettes (Sarstedt AG & Co., Nümbrecht, Germany), centrifuged at 13,000 × g for 7 min for serum collection and stored at −80°C until further use. Bone turnover markers were determined using RatLaps™ CTX-I EIA, Rat/Mouse PINP EIA (both from Immunodiagnostic Systems GmbH, Frankfurt am Main, Germany), mouse RANKL ELISA Kit (TNFSF11), and mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (both from Abcam, Cambridge, United Kingdom) according to manufacturers’ protocols. Using a customized mouse Multiplex Cytokine Kit (ProcartaPlex, eBiscience, Frankfurt, Germany), the CXCL-1, CXCL-10, IL-6, monocyte chemoattractant protein-1 (MCP-1), macrophage colony-stimulating factor (M-CSF), and vascular endothelial growth factor (VEGF) concentrations were determined in the OVX and Sham mice.
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