Antibody against ivl ma5 11803

Manufactured by Thermo Fisher Scientific

The Antibody against IVL (MA5–11803) is a laboratory reagent used for the detection and analysis of the IVL (Involucrin) protein. It is a monoclonal antibody produced by immunizing animals with a specific peptide sequence derived from the IVL protein. This antibody can be utilized in various immunochemical techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to identify and quantify the presence of IVL in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Antibodies against β actin clone w16197a, by BioLegend (2 mentions) Krt10 clone de k10, by BioLegend (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Antibodies against il, by R&D Systems (1 mentions)

2 protocols using antibody against ivl ma5 11803

Western Blot Analysis of Gene-Silenced NHPK Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Scrambled- and specific gene-siRNA–silenced differentiated NHPK Cells were lysed in 2× Laemmli sample buffer (Bio-Rad), and proteins were run on Western blots. Antibodies against β-actin (clone W16197A) and KRT10 (clone DE-K10) were purchased from BioLegend (San Diego, CA); antibody against IVL (MA5–11803) was purchased from Thermo Fisher Scientific (Carlsbad, CA); and antibody against LOR was a kind gift from Dr. Dennis Roop (University of Colorado).^{31 (link)} Antibodies against IL1β and IFNκ were purchased from R&D biosystems (Minneapolis, MN).

Bin L., Malley C., Taylor P., Boorgula M.P., Chavan S., Daya M., Mathias M., Shankar G., Rafaels N., Vergara C., Potee J., Campbell M., Hanifin J.M., Simpson E., Schneider L.C., Gallo R.L., Hata T., Paller A.S., De Benedetto A., Beck L.A., Ong P.Y., Guttman-Yassky E., Richers B., Baraghoshi D., Ruczinski I., Barnes K.C., Leung D.Y, & Mathias R.A. (2021). Whole genome sequencing identifies novel genetic mutations in patients with eczema herpeticum. Allergy, 76(8), 2510-2523.

+ Open protocol

+ Expand

Western Blot Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in 2x Laemmli sample buffer (Bio-Rad) and proteins were run on western blots. Antibodies against β-actin (clone W16197A) and KRT10 (clone DE-K10) were purchased from BioLegend; antibody against IVL (MA5-11803) was purchased from Thermo Scientific. Rabbit polyclonal anti-IFNκ (ab168119) was purchased from Abcam (Cambridge, MA).

Li Y., Song Y., Zhu L., Wang X., Richers B., Leung D.Y, & Bin L. (2020). Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1. Journal of Immunology Research, 2020, 5084682.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!