The cell culture supernatant was collected after 48 h of culture in serum-free medium. The supernatant was concentrated using Vivaspin® Turbo 15 (Sartorius Stedim Biotech GmbH, Goettingen, Germany). FITC-labeled precast gelatin gels and buffers for gelatin zymography were used in combination with the FITC-labeled Gelatin-zymography Kit (Cosmo Bio, Tokyo, Japan), according to the manufacturer's instructions. Briefly, the samples (2.5 μg) on gel-plates were electrophoresed at a constant current of 15 mA; the gels were then rinsed with wash buffer for 1 h. The enzyme reaction was performed by incubating the gels with an enzyme reaction buffer at 37°C for 24 h. The zymogram was imaged using the ChemiDoc XRS + system (Bio-Rad Laboratories) at an excitation wavelength of 535 mm.
Fitc labeled gelatin zymography kit
Manufactured by Cosmo Bio
Sourced in Japan
The FITC-labeled Gelatin-zymography Kit is a laboratory tool used for the detection and quantification of matrix metalloproteinases (MMPs) in biological samples. The kit contains fluorescein isothiocyanate (FITC)-labeled gelatin, which serves as a substrate for the enzymatic activity of MMPs. This technique allows for the visualization and analysis of MMP activity in a gel-based assay.
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Lab products found in correlation
2 protocols using fitc labeled gelatin zymography kit
1
Gelatin Zymography of Cell Culture Supernatant
2
Gelatin zymography of cell secretome
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The cell culture supernatant was collected after 48 h of culture in serum-free medium. The supernatant was concentrated using Vivaspin® Turbo 15 (Sartorius Stedim Biotech GmbH, Goettingen, Germany). FITC-labeled precast gelatin gels and buffers for gelatin zymography were used in combination with the FITC-labeled Gelatin-zymography Kit (Cosmo Bio, Tokyo, Japan), according to the manufacturer's instructions. Brie y, the samples (2.5 μg) on gel-plates were electrophoresed at a constant current of 15 mA; the gels were then rinsed with wash buffer for 1 h. The enzyme reaction was performed by incubating the gels with an enzyme reaction buffer at 37°C for 24 h. The zymogram was imaged using the ChemiDoc XRS + system (Bio-Rad Laboratories) at an excitation wavelength of 535 mm.
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