All chemicals used in the experiment were analytical grade and used as received. α–D–Glucose, A.C.S. reagent grade, glucose oxidase (GOx) from Aspergillus niger and horseradish peroxidase (HRP) were purchased from Sigma-Aldrich (St. Louis, MO). Potassium iodide was obtained from EM Science (Gibbstown, NJ). D-(+)-trehalose anhydrous was obtained from Tokyo Chemical Industry (Philadelphia, PA). Sodium phosphate monobasic anhydrous and sodium phosphate dibasic anhydrous were acquired from Fisher Scientific (Waltham, MA). All solutions were made in ultrapure water (18 MΩ cm−1, Barnstead Nanopure; Dubuque, IA). The paper substrates selected for these experiments (see Table 1 ) were purchased from Whatman (Maidstone, Kent, UK).
Sodium phosphate monobasic anhydrous
Manufactured by Thermo Fisher Scientific
Sourced in France
Sodium phosphate monobasic anhydrous is a chemical compound with the formula NaH2PO4. It is a white crystalline solid that is commonly used as a buffer in laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (6 mentions)
Sodium phosphate dibasic anhydrous, by Thermo Fisher Scientific (4 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (3 mentions)
Ethanol, by Thermo Fisher Scientific (2 mentions)
Sinigrin monohydrate, by Merck Group (2 mentions)
Tetraheptylammonium bromide, by Thermo Fisher Scientific (2 mentions)
Bradford reagent, by Avantor (2 mentions)
Horseradish peroxidase hrp, by Merck Group (1 mentions)
Orion 420a, by Thermo Fisher Scientific (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Thermo Fisher Scientific (1 mentions)
Potassium phosphate, by Merck Group (1 mentions)
Cesium chloride, by Merck Group (1 mentions)
Sodium phosphate dibasic heptahydrate, by Thermo Fisher Scientific (1 mentions)
Lithium chloride (licl), by Thermo Fisher Scientific (1 mentions)
Rubidium chloride, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Sodium sulfate, by Merck Group (1 mentions)
10 protocols using sodium phosphate monobasic anhydrous
1
Colorimetric Glucose Assay on Paper
2
Diazepam Pulsed Release Otic Formulation
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Example 23
Diazepam is used to prepare a pulsed release otic agent composition using the procedures described herein. A 17% poloxamer solution is prepared by dissolving 351.4 mg of sodium chloride (Fisher Scientific), 302.1 mg of sodium phosphate dibasic anhydrous (Fisher Scientific), 122.1 mg of sodium phosphate monobasic anhydrous (Fisher Scientific) and an appropriate amount of an otic agent with 79.3 g of sterile filtered DI water. The solution is cooled down in a ice chilled water bath and then 17.05 g of poloxamer 407NF (SPECTRUM CHEMICALS) is sprinkled into the cold solution while mixing. The mixture is further mixed until the poloxamer is completely dissolved. The pH for this solution is measured. 20% of the delivered dose of diazepam is solubilized in the 17% poloxamer solution with the aid of beta-cyclodextrins. The remaining 80% of the otic agent is then added to the mixture and the final composition is prepared using any procedure described herein.
Pulsed release compositions comprising Compositions comprising collagen, keratin, collagenase, or micronized collagen prepared according to the procedures and examples described herein, are tested using procedures described herein to determine pulse release profiles.
3
Diazepam Pulsed Release Otic Composition
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Example 10
Diazepam is used to prepare a pulsed release otic agent composition using the procedures described herein. A 17% poloxamer solution is prepared by dissolving 351.4 mg of sodium chloride (Fisher Scientific), 302.1 mg of sodium phosphate dibasic anhydrous (Fisher Scientific), 122.1 mg of sodium phosphate monobasic anhydrous (Fisher Scientific) and an appropriate amount of an otic agent with 79.3 g of sterile filtered DI water. The solution is cooled down in a ice chilled water bath and then 17.05 g of poloxamer 407NF (SPECTRUM CHEMICALS) is sprinkled into the cold solution while mixing. The mixture is further mixed until the poloxamer is completely dissolved. The pH for this solution is measured. 20% of the delivered dose of diazepam is solubilized in the 17% poloxamer solution with the aid of beta-cyclodextrins. The remaining 80% of the otic agent is then added to the mixture and the final composition is prepared using any procedure described herein.
Pulsed release compositions comprising Compositions comprising alprazolam, clonazepam, diazepam, or micronized diazepam prepared according to the procedures and examples described herein, are tested using procedures described herein to determine pulse release profiles.
4
Lysozyme Adsorption Characterization
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All aqueous solutions were prepared using 18 MΩ⋅cm water (NANOpure Diamond, Barnstead; Dubuque, IA) and analytical grade chemicals. Lysozyme (LSZ, from chicken egg white) was purchased from Sigma‐Aldrich (St. Louis, MO). Sodium phosphate monobasic anhydrous was obtained from Fisher Scientific (Fair Lawn, NJ). AZ P4330‐RS Photoresist was acquired from AZ Electronic Materials USA Corp. (Somerville, NJ). Propylene glycol monomethyl ether acetate (PGMEA 99 %) was obtained from Alfa Aesar (Ward Hill, MA).
Stock solutions of LSZ (0.10 mg.mL−1) were prepared daily by dissolving a known amount of protein in 10 mmol.L−1 phosphate buffer solution at pH=11.00 (IEP, isoelectric point). The latter aspect is important as this pH value avoids potential contribution of (additional) charged residues on the overall dielectric behavior of proteins.[42 , 43 ] The pH of buffer solution was adjusted using 1 mol.L−1 NaOH and measured with a glass electrode connected to a digital pH meter (Orion 420A+, Thermo; Waltham, MA). Before adsorption, the protein solution was filtered through 0.2 μm poly(tetrafluoroethylene) membrane (PTFE, VWR International; Radnor, PA) to remove any aggregates.
Stock solutions of LSZ (0.10 mg.mL−1) were prepared daily by dissolving a known amount of protein in 10 mmol.L−1 phosphate buffer solution at pH=11.00 (IEP, isoelectric point). The latter aspect is important as this pH value avoids potential contribution of (additional) charged residues on the overall dielectric behavior of proteins.[
5
Recombinant Albumin Reagent Preparation
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Bovine serum albumin, sucrose, potassium chloride, rubidium chloride, cesium chloride, potassium phosphate, and sodium sulfate were purchased from Sigma-Aldrich (St. Louis, MO). Lithium chloride, sodium chloride, sodium phosphate monobasic anhydrous, sodium phosphate dibasic heptahydrate, and potassium phosphate monobasic were purchased from Fisher Scientific (Pittsburgh, PA). Recombinant HSA in sterile ultrahigh purity grade was a product of Albumin Bioscience (Huntsville, AL).
6
Synthesis and Characterization of SU-101
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All reagents were purchased and used without further purification. Ellagic acid (EA) reagent-grade (ACROS Organics, 97%), bismuth (III) acetate (Alfa Aesar, 99%), and glacial acetic acid (Merck, 100%) were used for the synthesis of SU-101. PhACs, used for the adsorption and photodegradation experiments, include atenolol (At; Sigma-Aldrich, ≥ 98%), sulfamethazine (SMT; Sigma-Aldrich, ≥ 99%) and sodium diclofenac (DCF; Sigma-Aldrich, ≥ 98). Formic acid (FA; Thermo Scientific, ≥ 98%) and acetonitrile (ACN; J.T. Baker, HPLC-grade) were used for the preparation of HPLC mobile phases. For the preparation of the phosphate buffered saline (PBS) solution, sodium anhydrous phosphate dibasic (ACROS Organics, 98%), sodium phosphate monobasic anhydrous (ACROS Organics, 98%), orthophosphoric acid (ACROS Organics, ≥ 85%) and Milli-Q water were used. Isopropyl alcohol (VWR, 99%), p-benzoquinone (ACROS, ≥ 98%), disodium ethylenediaminetetraacetate dihydrate (Na2EDTA, Fisher Scientific, 99.5%) and silver nitrate (ACROS Organics, 99.9%) were employed for active species trapping tests.
7
Graphene Oxide-based Photocatalytic System
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All the reagents were commercially available and used as purchased. All water-based solutions were prepared using either distilled or Milli q water. Graphene oxide (GO) was prepared by a reported procedure [40 (link)]. Methylene blue (MB) and copper nitrate were purchased from Sigma-Aldrich; sodium phosphate, monobasic, anhydrous from Acros Organics; di-Sodium hydrogen phosphate anhydrous from VWR Chemicals, and titanium dioxide (TiO2-P25) from Evonik.
8
Biomolecule Immobilization Protocol
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(AAm) (99.9%), 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide HCl (EDC), N-hydroxysuccinimide (NHS), 2-(4morpholino)ethanesulfonic acid (MES), hexadecane, 2-propanol, sodium phosphate monobasic anhydrous (99%), sodium phosphate dibasic anhydrous (≥ 99%), Tween 20, poly(dimethylsiloxane) (PDMS) elastomer kits (Sylgard 184) and sodium hydroxide (NaOH) were purchased from Thermo Fisher Scientific (Waltham, MA). Acrylic acid (AAc) anhydrous (180-200ppm MEHQ inhibitor,99%), 2-hydroxy-2-methylpropiophenone (Darocur 1173, photoinitiator), saline sodium citrate (SSC) buffer (20× concentrate, molecular biology grade), N-Boc ethylene diamine, hydroxybenzotriazole (HOBt), N,N-dimethylformamide (DMF), Tetrahydrofuran (THF), N,N'-Diisopropylcarbodiimide (DIC), cis-dichlorobis(2,2'-bipyridine)ruthenium(II), N,N'-Methylenebis(acrylamide) (Bis) were purchased from Sigma Aldrich.
4-Methyl-4'carboxy-2,2'-bipyridine was purchased from Carbosynth. All chemicals were of analytical grade and used without further purification.
4-Methyl-4'carboxy-2,2'-bipyridine was purchased from Carbosynth. All chemicals were of analytical grade and used without further purification.
9
Sinigrin and Glucotropeoalin Extraction
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Sinigrin monohydrate was purchased from Sigma Aldrich (Saint-Quentin Fallavier, France).
Glucotropeoalin was provided by Terres Inovia (Ardon laboratory, France). Acetonitrile, ethanol, tetraheptylammonium bromide, sodium phosphate monobasic anhydrous were obtained from Fischer Scientific (Illkirch, France). Bradford reagent was provided by VWR (Strasbourg, France).
Glucotropeoalin was provided by Terres Inovia (Ardon laboratory, France). Acetonitrile, ethanol, tetraheptylammonium bromide, sodium phosphate monobasic anhydrous were obtained from Fischer Scientific (Illkirch, France). Bradford reagent was provided by VWR (Strasbourg, France).
10
Sinigrin and Glucotropeoalin Extraction
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Sinigrin monohydrate was purchased from Sigma Aldrich (Saint-Quentin Fallavier, France).
Glucotropeoalin was provided by Terres Inovia (Ardon laboratory, France). Acetonitrile, ethanol, tetraheptylammonium bromide, sodium phosphate monobasic anhydrous were obtained from Fischer Scientific (Illkirch, France). Bradford reagent was provided by VWR (Strasbourg, France).
Glucotropeoalin was provided by Terres Inovia (Ardon laboratory, France). Acetonitrile, ethanol, tetraheptylammonium bromide, sodium phosphate monobasic anhydrous were obtained from Fischer Scientific (Illkirch, France). Bradford reagent was provided by VWR (Strasbourg, France).
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