Cellular suspensions were loaded on the Chromium™ Controller (10x Genomics, Pleasanton, CA, USA) in order to generate Gel Bead-In-EMulsions (GEMs). Barcoded sequencing libraries were generated by using the Chromium™ Single Cell 3’ Reagent Kits v2 (10x Genomics, Pleasanton, CA, USA) following manufacturer’s instructions. Sequencing libraries were loaded at 1.4 pM on an Illumina NextSeq500 with NextSeq 500/550 Mid Output v2 kit (150 cycles) (Illumina, CA, USA) using the following read lengths: 26 bp for Read1 (16 bp Barcode + 10 bp Randomer), 8 bp for Sample Index and 58 bp for Read2.
Nextseq500 with nextseq 500 550 mid output v2 kit
Manufactured by Illumina
Sourced in United States
The NextSeq 500 is a high-throughput sequencing system designed for a wide range of applications, from targeted resequencing to whole-genome analysis. The NextSeq 500/550 Mid Output v2 kit is a sequencing reagent kit compatible with the NextSeq 500 system, providing users with the necessary consumables to perform mid-output sequencing runs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nextseq500 with nextseq 500 550 mid output v2 kit
1
Single-cell RNA-seq library preparation
2
Single-cell RNA-seq library preparation
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