The cell lines, including B lymphoma Maver-1, mantle cell lymphoma Jeko-1, Burkitt's lymphoma CA46, acute erythroid leukemia HEL, diffuse histiocytic lymphoma SU-DHL-1 and breast cancer MDA-MB-468 were purchased from ATCC (Manassas, VA). All cell suspensions were cultured in RPMI 1640 medium (Thermo Fisher Scientific, Rockford, IL) containing 10% Fetal Bovine Serum (FBS, Atlanta Biologicals, Lawrenceville, GA), and 100 IU/mL penicillin-streptomycin. All adhesion cell lines were cultured with DMEM (Atlanta Biologicals, Lawrenceville, GA) containing 10% FBS and 100 IU/mL penicillin-streptomycin. The washing buffer used during aptamer enrichment contained 4.5 g/L glucose and 5 mmol/L MgCl2 in Dulbecco's Phosphate-Buffered Saline (DPBS, Sigma Aldrich, St. Louis, MO). Binding buffer was prepared by adding Bovine Serum Albumin (1 mg/mL; BSA, Thermo Fisher Scientific) with 0.1 mg/mL t-RNA (Sigma Aldrich) to the washing buffer.
Su dhl 1
Manufactured by American Type Culture Collection
The SU-DHL-1 is a laboratory equipment designed for cell culture applications. It is a suspension culture device used for the growth and maintenance of cells in a liquid medium. The SU-DHL-1 provides a controlled environment for cell cultivation, enabling efficient cell expansion and biomass production.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Sr 786, by American Type Culture Collection (2 mentions)
Karpas 299, by American Type Culture Collection (2 mentions)
Sup m2, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Atlanta Biologicals (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Merck Group (1 mentions)
Maver 1, by American Type Culture Collection (1 mentions)
Jeko 1, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Su dhl 6, by American Type Culture Collection (1 mentions)
Su dhl 4, by American Type Culture Collection (1 mentions)
Roswell park memorial institute (rpmi), by Corning (1 mentions)
Colo205, by American Type Culture Collection (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Vincristine, by Merck Group (1 mentions)
4 hydroperoxycyclophosphamide 4hc, by Merck Group (1 mentions)
Anti mouse and anti rabbit secondary antibodies, by Bio-Rad (1 mentions)
7 protocols using su dhl 1
1
Cell Culture Conditions for Aptamer Selection
2
Authentication and Culture of Human Cell Lines
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The Ramos (RRID: CVCL_0597), SUDHL4 (CVCL_0539), Raji (CVCL_0511), Daudi (CVCL_0008), SUDHL6 (CVCL_2206), Namalwa (CVCL_0067), Jurkat (CVCL_0367), SUDHL1 (CVCL_0538), SR-786 (CVCL_1711), and U937 (CVCL_0007) human cell lines were obtained from ATCC and were used within 3 months of receipt and/or resuscitation. ATCC uses short tandem repeat (STR) profiling to authenticate their cell lines prior to shipping. For SUDHL4 cells, Charles River Laboratories was contracted to test for mycoplasma contamination prior to use in animal experiments. All cell lines were cultured in RPMI (Corning) 1640 supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37 °C in a humidified, 5% CO2 incubator.
3
Spiking Cell Lines into Donor Blood
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Authenticated cell line cells H820 (lung cancer), Colo205 (colon cancer), A549 (lung cancer), SU-DHL-1 (lymphoma), H441 (lung cancer) and H23 (lung cancer), were purchased from ATCC and cultured in RPMI 1640 media supplemented with 10 % fetal bovine serum. Where applicable, cells were treated for 24 h with 100 ng/mL IFN-γ (R&D Systems, Minneapolis, MN). Cell line cells were then detached and spiked into healthy donor (HD) blood, which was then processed per Epic Sciences standard operating procedure [28 , 29 ]. Briefly, red blood cells were lysed using ammonium chloride solution and the remaining nucleated cells were plated onto glass slides at a density of 3 million cells per slide. Slides were then stored in a − 80 °C biorepository until used for immunofluorescence (IF) staining and analysis.
4
Cytotoxic Drug Combination Protocol
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4-Hydroperoxy-cyclophosphamide (4-HC, the active metabolite of cyclophosphamide), vincristine and doxorubicin, which compose the CHO treatment (2 µM 4-HC, 20 nM doxorubicin; 0.5 nM vincristine), were purchased from SIGMA-Aldrich (St. Louis, MO, USA). All other drugs were from Selleck Chem (Houston, TX, USA). For in vitro analyses, drugs were dissolved in DMSO; for in vivo studies, crizotinib and trametinib were prepared fresh in 0.5% carboxymethylcellulose/0.1% Tween 80; all the other compounds were dissolved in PBS. Primary antibodies (Table S1 ) were diluted 1:1000 in 5% BSA and incubated overnight. Anti-mouse and anti-rabbit secondary antibodies (Bio-Rad, Hercules, CA, USA) were used 1:2000 in 5% BSA. ALK+ ALCL cell lines Karpas-299, SUP-M2 and SU-DHL-1, and ALK-negative myelomonocytic leukemia U937 cells were purchased from ATCC. Cells were cultured in RPMI-1640 medium (Euroclone, Siziano, Italy) supplemented with 10% OptiClone fetal bovine serum, 2 mM L-glutamine, 100 units/mL penicillin G, 80 μg/mL gentamicin and 20 mM HEPES, in a humidified atmosphere at 37 °C and 5% CO2.
5
ALCL Cell Lines Viability Assay
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ALCL cell lines Karpas299, SUDHL1, JB6, SR-786, SUP-M2, FE-PD, MAC1, and MAC2A were obtained from ATCC. All cell lines were cultured in RPMI-1640 supplemented with 10% FBS and 100 IU/mL penicillin. Cells were tested for mycoplasma every 3 months with the Mycoalert kit (Promega), and identity was confirmed by STR profiling (DFCI molecular diagnostics laboratory). Cell proliferation assays were performed by seeding 5000 cells per well in 96-well plates containing DMSO, crizotinib, alectinib, iberidomide, Stattic, or STAT3-IN-3 (MedChemExpress, LLC). Cell viability was assayed with CellTiter-Glo according to the manufacturer’s protocol (Promega).
6
Cell Culture Protocol for Hematological Malignancies
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CCRF-CEM, HH, HUT-78, LOUCY, MJ, MOLT-4, SU-DHL-1 and SUP-T1 cell lines were purchased from the American Type Culture Collection (ATCC) and cultured with Roswell Park Memorial Institute (RPMI) 1640 Medium (Thermo Fisher Scientific) containing 10% fetal bovine serum (Gibco), except for the MJ cell line, which was cultured with Gibco Iscove’s Modified Dulbecco’s Media (IMDM) medium (Thermo Fisher Scientific) containing 20% fetal bovine serum.
BE-13, DND-41, HD-MAR-2, HPB-ALL, HSB-2, JURKAT, KE-37, L-82, MHH-TALL-2, MOLT-14, MOLT-16, P12-ICHIKAWA, PF-382, RPMI-8402, SR-786 and TALL-1 were purchased from the DSMZ German Collection of Microorganisms and Cell Cultures. Except for HSB-2, they were cultured with Roswell Park Memorial Institute (RPMI) 1640 Medium containing different proportions of fetal bovine serum: 10% for DND-41, HD-MAR-2, HPB-ALL, JURKAT, KE-37, L-82, MOLT-14, MOLT-16, P12-ICHIKAWA, PF-382 and RPMI-8402; 20% for BE-13 and MHH-TALL-2; 15% for SR-786 and TALL-1. HSB-2 was cultured with Gibco Iscove’s Modified Dulbecco’s Media (IMDM) medium containing 10% fetal bovine serum.
MyLa was purchased from the European Collection of Authenticated Cell Cultures (ECACC) and was cultured with Roswell Park Memorial Institute (RPMI) 1640 Medium containing 10% fetal bovine serum.
All cells were cultured in CO2 incubators at 37 °C in an atmosphere containing 5% CO2.
BE-13, DND-41, HD-MAR-2, HPB-ALL, HSB-2, JURKAT, KE-37, L-82, MHH-TALL-2, MOLT-14, MOLT-16, P12-ICHIKAWA, PF-382, RPMI-8402, SR-786 and TALL-1 were purchased from the DSMZ German Collection of Microorganisms and Cell Cultures. Except for HSB-2, they were cultured with Roswell Park Memorial Institute (RPMI) 1640 Medium containing different proportions of fetal bovine serum: 10% for DND-41, HD-MAR-2, HPB-ALL, JURKAT, KE-37, L-82, MOLT-14, MOLT-16, P12-ICHIKAWA, PF-382 and RPMI-8402; 20% for BE-13 and MHH-TALL-2; 15% for SR-786 and TALL-1. HSB-2 was cultured with Gibco Iscove’s Modified Dulbecco’s Media (IMDM) medium containing 10% fetal bovine serum.
MyLa was purchased from the European Collection of Authenticated Cell Cultures (ECACC) and was cultured with Roswell Park Memorial Institute (RPMI) 1640 Medium containing 10% fetal bovine serum.
All cells were cultured in CO2 incubators at 37 °C in an atmosphere containing 5% CO2.
7
Cell Lines for ALK-Positive ALCL Research
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Two ALK-positive ALCL cell lines were used in this study: Karpas 299 was obtained from Sigma-Aldrich (St. Louis, MO) and SU-DHL-1 from American Type Culture Collection (Manassas, VA). NCI-H2228, an EML4-ALK–positive lung adenocarcinoma cell line, was obtained from American Type Culture Collection (Manassas, VA). Cells were maintained in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (GIBCO, Grand Island, NY) and 1% penicillin/streptomycin (Lonza, Walkersville, MD). All cells were incubated at 37°C in an atmosphere of 5% CO2.
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