Rps6 2217

Manufactured by Cell Signaling Technology

Sourced in United States

RPS6 (2217) is a primary antibody that recognizes the ribosomal protein S6 (RPS6). RPS6 is a component of the 40S ribosomal subunit and is involved in the regulation of protein synthesis.

Automatically generated - may contain errors

Lab products found in correlation

Phalloidin fitc p5282, by Merck Group (1 mentions) Vdac 4866, by Cell Signaling Technology (1 mentions) Hnrnp u, by Abcam (1 mentions) Hdac1, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions)

2 protocols using rps6 2217

Probing Cellular Signaling Pathways

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Primary antibodies against phospho-MLC (3674), MLC (8505), phospho-mTOR (2971), mTOR (2983), α-tubulin (3873), GAPDH (2118), phospho-RPS6 (4585), and RPS6 (2217); the RhoA Activation Assay Kit (8789); and rapamycin (8789) were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC-Phalloidin (P5282) and Y-27632 (S1049) were purchased from Sigma Aldrich (Saint Louis, MO, USA) and Med Chem Express (Newark, NJ, USA), respectively.

Chen X., Chen J., Li X, & Yu Z. (2021). Activation of mTOR mediates hyperglycemia-induced renal glomerular endothelial hyperpermeability via the RhoA/ROCK/pMLC signaling pathway. Diabetology & Metabolic Syndrome, 13, 105.

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Quantifying Protein Expression in Cellular Immunostaining

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Cells were sorted, seeded on coverslips, fixed, immunostained, and the intensity quantified as described in Iborra and Buckle (2008) (link).
In Figures 1, 2, and 3, the protein antibodies used were MTOR(2983), PFKP(12746), FASN(3180), HK2(2867), LDHA(2012), PRPS6(4858) (Cell Signaling); SF3B4(AB66659), HNRNPU(AB20666), NFE2L2(AB31163), EPRS(AB31531), RRS(AB31537), HK4me3(AB8580) (Abcam); HNRNPA0(SC-16509), EP300(SC-584), TBP(SC-56794), GTF2F(SC-235), PKM2(SC-365684), UBTF(SC-13125), RNA Pol II(RPB1)(SC-9001) (Santa Cruz Biotechnologies); CLU(HPA000572), SRSF2(HPA049905), MCL1(HPA008455), TUBB(T8328), G6PD(HPA000834), PDIA2(HPA051692), GAPDH(G9545), PTBP1(WH0005725M1) (Sigma-Aldrich); GLUT1(AB1340) (Chemicon); BrU(MD5010) (Caltag); H4K20me2(39173), H3K36me2(39255), H4K16(39929), H3K27me3(61017) (Active Motifs); and PABP (kindly provided by Dr. Amelia Nieto).
For Figure 5B, the 24 antibodies used for protein quantification were HK2(2867), HDAC1(5356), LDHA(2012), RPS6(2217), EIF4B(13088), VDAC(4866), PFKP(12746) (Cell Signaling); G6PD(HPA000834), MCL1(HPA008455), TOMM22(HPA003037), CLU(HPA000572), PTBP1(WH0005725M1) (Sigma-Aldrich); NPM1(SC-6013), NCL(SC-8031), UBTF(SC-13125), PKM2(SC-365684), GTF2F(SC-235), BCL2(SC-492) (Santa Cruz Biotechnology); NFE2L2(AB31163), SF3B4(AB66659), IMMT(AB110329) (Abcam); GLUT1(AB1340) (Chemicon); EIF5(611976), EIF6(611120) (BD).

Guantes R., Rastrojo A., Neves R., Lima A., Aguado B, & Iborra F.J. (2015). Global variability in gene expression and alternative splicing is modulated by mitochondrial content. Genome Research, 25(5), 633-644.

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