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The TIB 160 is a compact, benchtop incubator designed for applications that require precise temperature control. It features a temperature range of 5°C above ambient to 60°C and can maintain temperature stability within ±0.1°C. The TIB 160 is suitable for a variety of applications that require consistent and reliable temperature conditions.

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2 protocols using tib 160

1

CFSE-based Cytotoxicity Assay for Yac 1 Cells

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Yac 1 cell line (ATCC® - TIB 160) was cultivated in RPMI 1640 medium (ATCC® 30–2001) supplemented with 10% Fetal bovine serum (FBS, ATCC® 30–2020) and kept with air, 95%; carbon dioxide (CO2), 5% at 37ºC. Yac 1 cells were expanded for a week then counted on a Neubauer chamber and finally stained with CFSE kit for further use in cytotoxic assays.
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2

Culturing Mammary and Leukemia Cell Lines

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Mouse mammary gland cells, 4 T1 (CRL-2539, ATCC, USA), human mammary gland cells MDA-MB-231 (HTB-26, ATCC, USA) and murine leukemia virus induced YAC-1 (TIB-160, ATCC, USA) were purchased from the ATCC collection and cultured in RPMI 1640 (R8758, Sigma Aldrich, USA) containing 10% fetal bovine serum (FBS) (26140, Gibco, USA). The cells were grown at 37 °C in a humidified incubator with 5% CO2.
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