Anti rabbit dnajb6

Manufactured by Abcam

Anti-rabbit DNAJB6 is a laboratory reagent used in research applications. It is a protein that binds to and interacts with the DNAJB6 protein, which is involved in various cellular processes. The primary function of this product is to facilitate the study of DNAJB6 and its role in biological systems.

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Lab products found in correlation

Rabbit anti gapdh, by Cell Signaling Technology (3 mentions) Mouse anti desmin, by Agilent Technologies (2 mentions) Anti rabbit αβ crystallin, by Enzo Life Sciences (2 mentions) Anti rabbit gsk3β, by Cell Signaling Technology (1 mentions) Mouse anti flag, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-DNAJB6 Rabbit anti-

3 protocols using anti rabbit dnajb6

Characterization of C2C12 Myotube Proteins

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Antibodies used were the following: anti-rabbit GAPDH (Cell Signaling, 2118), anti-mouse Desmin (Dako, M0760), anti-rabbit DNAJB6 (Abcam, ab198995), anti-mouse HspA1 (Enzo, ADI-SPA-810), anti-rabbit α-actinin (Abcam, ab68167), anti-rabbit Myotilin (Abcam, ab78492), anti-rabbit Synemin (Bioss, bs-8555R), anti-rabbit αβ-crystallin (Enzo, ADI-SPA-223), anti-rabbit DNAJB4 (proteintech, 13064–1-AP) and anti-human DNAJB4 (Santa Cruz, sc-100711). Rhodamine Phalloidin Reagent (Invitrogen, PHDR1) was used to stain actin filaments in C2C12 myotubes for fluorescence microscopy. Secondary antibodies include anti-mouse HRP (Pierce), anti-rabbit HRP (Cell Signaling) anti-rabbit AlexaFluro (555 and 488).

Weihl C.C., Töpf A., Bengoechea R., Duff J., Charlton R., Garcia S.K., Domínguez-González C., Alsaman A., Hernández-Laín A., Franco L.V., Sanchez M.E., Beecroft S.J., Goullee H., Daw J., Bhadra A., True H., Inoue M., Findlay A.R., Laing N., Olivé M., Ravenscroft G, & Straub V. (2022). Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure. Acta neuropathologica, 145(1), 127-143.

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Immunoblot Analysis of Muscle Proteins

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Antibodies used were the following: anti-rabbit GAPDH (Cell Signaling, 2118), anti-rabbit desmin (Abcam, ab8592), anti-rabbit DNAJB6 (Abcam, ab75196), anti-rabbit αβ-crystallin (Enzo, ADI-SPA-223), anti-mouse hnRNPA2/B1 (Sigma, R4653), anti-rabbit alpha-actinin (Abcam ab68167), anti-mouse keratin 18 (Abcam, ab668), anti-rabbit GSK3β-P(ser-9) (Cell Signaling, 9336), anti-rabbit GSK3β (Cell Signaling, 9315), anti-goat FHL-1 (Abcam ab23937), and anti-mouse myosin (Sigma-Aldrich, M1570). Secondary antibodies include anti-mouse HRP (Pierce), anti-rabbit HRP (Cell Signaling), anti-goat HRP (Santa Cruz), and anti-mouse AlexaFluor (488).

Findlay A.R., Bengoechea R., Pittman S.K., Chou T.F., True H.L, & Weihl C.C. (2019). Lithium chloride corrects weakness and myopathology in a preclinical model of LGMD1D. Neurology: Genetics, 5(2), e318.

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Multiplex Antibody Validation Protocol

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Antibodies used were the following: anti-rabbit Sis1 (Cosmo Bio Co., ltd., COP-080051), anti-mouse Pgk1 (Abcam, 113687), anti-rabbit GAPDH (Cell Signaling, 2118), anti-V5 HRP Invitrogen (B96125), anti-mouse Desmin (Dako, M0760), anti-rabbit TDP43 (Proteintech, 10782-2-AP), anti-rabbit DNAJB6 (abcam, ab198995), anti-mouse Hsp70 (Enzo, ADI-SPA-812), anti-rabbit P62 "SQSTM1" (Proteintech, 18420-1-AP), anti-rabbit laminin (ab1575), anti-rabbit GFP (Sigma g1544), anti-mouse Flag (Sigma F3165). Secondary antibodies include anti-mouse HRP (Pierce), anti-rabbit HRP (cell signaling) anti-rabbit AlexaFluro (555 and 488).

Bengoechea R., Findlay A., Bhadra A.K., Shao H., Stein K.C., Pittman S.K., Daw J., Gestwicki J.E., True H.L., & Weihl C.C. (2020). Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy.

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