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Annexin 5 fluorescein isothiocyanate annexin 5 fitc apoptosis detection kit

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The Annexin V-fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death, in cell populations. The kit utilizes annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells. The annexin V is labeled with the fluorescent dye FITC, allowing for the visualization and measurement of apoptotic cells using flow cytometry or fluorescence microscopy.

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Facs analysis, by BD (2 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit Annexin-V-fluorescein-5-isothiocyanate (Annexin-V-FITC) apoptosis detection kit Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit I Fluorescein isothiocyanate-conjugated (FITC) anti-human Annexin V Apoptosis Detection Kit I BD Pharmingen® fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit II Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit Fluorescein isothiocyanate (FITC)-conjugated Annexin V Apoptosis Detection kit Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit Annexin V–fluorescein isothiocyanate (FITC)-labeled Apoptosis Detection Kit I

4 protocols using annexin 5 fluorescein isothiocyanate annexin 5 fitc apoptosis detection kit

1

Measuring Apoptosis in Cell Lines

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The cells (4 × 105) were seeded in 35 mm dishes containing 7 mL of complete medium and exposed to increasing concentrations of GMX-1778 and STF-118804 for 48 hr. Apoptosis was measured as previously described (36) using the annexin V fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit (BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions. Following the treatment, the annexin positive cells were quantitated via FACS analysis (Becton Dickinson, Ranklin Lakes, NJ, USA) using FCS Express Cytometry Software (Tree Star, Inc., Ashland, OR, USA). The percentage of annexin positive cells was calculated and the delta between the treated and untreated control was determined.
Safari M., Scotto L., Litman T., Petrukhin L.A., Zhu H., Shen M., Robey R.W., Hall M.D., Fojo T, & Bates S.E. (2023). Novel Therapeutic Strategies Exploiting the Unique Properties of Neuroendocrine Neoplasms. Cancers, 15(20), 4960.
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2

Measurement of Apoptosis by Annexin V-FITC

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Apoptosis was measured using the annexin V-fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit (BD Biosciences, San Diego, CA) according to the manufacturer’s instructions. Cells were treated with 25 ng/ml (45 nM) romidepsin or 25 μM vorinostat for 6 hours, washed, and incubated an additional 42 hours in drug-free medium. Following treatment, annexin positive cells were quantitated by FACS analysis (Becton Dickinson, Ranklin Lakes, NJ) using FlowJo Software (Tree Star, Inc, Ashland, OR). The percentage of annexin positive cells was derived, and the delta between treated and untreated control determined.
Luchenko V.L., Litman T., Chakraborty A.R., Heffner A., Devor C., Wilkerson J., Stein W., Robey R.W., Bangiolo L., Levens D, & Bates S.E. (2014). Histone deacetylase inhibitor‐mediated cell death is distinct from its global effect on chromatin. Molecular Oncology, 8(8), 1379-1392.
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3

Annexin V-FITC Apoptosis Detection

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Apoptosis was measured as previously described (19 (link)) using the annexin V-fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit (BD Biosciences, San Diego, CA) according to the manufacturer’s instructions. Following treatment, annexin positive cells were quantitated by FACS analysis (Becton Dickinson, Ranklin Lakes, NJ) using FlowJo Software (Tree Star, Inc, Ashland, OR) and FCS Express 7 Software (De Novo Software Inc.). The percentage of annexin positive was calculated and the delta between treated and untreated control was determined.
Safari M., Litman T., Robey R.W., Aguilera A., Chakraborty A.R., Reinhold W.C., Basseville A., Petrukhin L., Scotto L., O'Connor O.A., Pommier Y., Fojo A.T, & Bates S.E. (2021). R-Loop–Mediated ssDNA Breaks Accumulate Following Short-Term Exposure to the HDAC Inhibitor Romidepsin. Molecular Cancer Research, 19(8), 1361-1374.
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4

Annexin V-FITC/PI Apoptosis Assay for NSCLC

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Annexin V‐fluorescein isothiocyanate (Annexin V‐FITC) Apoptosis Detection Kit (BD Biosciences) was used to analyze the proportion of NSCLC cells with FITC+ and propidium iodide+/− (PI+/−). NSCLC cells after 72 h of transfection were incubated with Annexin V‐FITC (5 μl) and PI (5 μl) for 15 min in the dark. Cell apoptosis was assessed on FACSCalibur (BD Biosciences).
Chen S., Zhou L., Ran R., Huang J., Zheng Y., Xing M, & Cai Y. (2021). Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro. Thoracic Cancer, 12(23), 3223-3235.
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