Custom antibody

Manufactured by Cell Signaling Technology

Custom antibodies are laboratory-produced proteins that can recognize and bind to specific targets, such as proteins or other biomolecules, in order to study their function and behavior. These antibodies are designed and manufactured to order based on the customer's requirements.

Automatically generated - may contain errors

Lab products found in correlation

Facscanto, by BD (1 mentions) Anti igg1 antibody, by Abcam (1 mentions) A0545, by Merck Group (1 mentions) Ap136p, by Merck Group (1 mentions) Lamin b sc6217, by Santa Cruz Biotechnology (1 mentions) Alpha tubulin t9026, by Merck Group (1 mentions) A5420, by Merck Group (1 mentions) Snail, by Cell Signaling Technology (1 mentions)

2 protocols using custom antibody

EpCAM and PD-L1 Expression Profiling

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Cancer cell lines (100,000 cells/tube) were incubated with antibodies against EpCAM (Miltenyi, Biotec, Custom antibody) and PD-L1 (XP version, Cell Signaling Danvers, MA) for 1 h on ice. Anti-IgG1 antibody was used as negative control (Abcam, Cambridge, UK). After 3 washes in PBS, samples were incubated with PE- or FITC-conjugated secondary antibodies for 1 h on ice. FACS analysis was performed by FACS-Canto instrument (BD, Becton-Dickinson, Franklin Lakes, NJ, USA).

Signore M., Alfonsi R., Federici G., Nanni S., Addario A., Bertuccini L., Aiello A., Di Pace A.L., Sperduti I., Muto G., Giacobbe A., Collura D., Brunetto L., Simone G., Costantini M., Crinò L., Rossi S., Tabolacci C., Diociaiuti M., Merlino T., Gallucci M., Sentinelli S., Papalia R., De Maria R, & Bonci D. (2021). Diagnostic and prognostic potential of the proteomic profiling of serum-derived extracellular vesicles in prostate cancer. Cell Death & Disease, 12(7), 636.

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Comprehensive Protein Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

NR2F2 Isoform 1 (41859, Abcam for western blot (1:1000)); 61214, Active Motif for ChIP (5 μL per sample), NR2F2 Isoform 2 (Millipore custom antibody (1:1000)), SNAIL (3879, Cell Signaling Technology (1:1000)), Actin (A3854, Sigma (1:50,000)), Lamin-B (sc6217, Santa Cruz (1:50,000)), Alpha-tubulin (T9026, Sigma (1:50,000)). Rabbit secondary (Sigma, A0545 (1:20,000)), mouse secondary (Sigma, A9044 (1:20,000)), rat secondary (Millipore, AP136P (1:20,000)), and goat secondary (Sigma, A5420 (1:20,000)).

Davalos V., Lovell C.D., Von Itter R., Dolgalev I., Agrawal P., Baptiste G., Kahler D.J., Sokolova E., Moran S., Piqué L., Vega-Saenz de Miera E., Fontanals-Cirera B., Karz A., Tsirigos A., Yun C., Darvishian F., Etchevers H.C., Osman I., Esteller M., Schober M, & Hernando E. (2023). An epigenetic switch controls an alternative NR2F2 isoform that unleashes a metastatic program in melanoma. Nature Communications, 14, 1867.

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