25 cm2 tc flasks

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The 25-cm2 TC flasks are culture vessels designed for the in vitro cultivation of cells. They provide a surface area of 25 square centimeters for cell attachment and growth. The flasks are made of clear, transparent polystyrene material and feature a vented cap to allow gas exchange.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Mco 17ai, by Sanyo (2 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

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25 cm2 flasks

2 protocols using 25 cm2 tc flasks

Culturing Mouse Neuroblastoma N2A Cells

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Experiments were carried out using mouse neuroblastoma N2A cell line (tumor-like neuroblasts), which were kindly provided by Dr. M. Mojarad. The cells were cultured either in 96-well tissue (TC) plate (NUNC, Wiesbaden, Germany) or in 25-cm² TC flasks (NUNC, Wiesbaden, Germany). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal calf serum (Gibco–Invitrogen), 100 IU·ml^-1 penicillin (Gibco–Invitrogen), and 100 μg·ml^-1 streptomycin (Gibco–Invitrogen). The cells were incubated in a humidified 5% CO₂ and 95% air atmosphere at 37°C in CO₂ incubator MCO-17AI (Sanyo Electric Co., Ltd., Japan). The culture medium was replaced 3 times a week. At the time of the experiment, confluent cells were trypsinized and plated in 96-well plates or into tissue culture dishes (40 mm in diameter; TPP). N2A cells were plated at a density of 31, 000 cells/cm². Experiments were initiated 72 h after plating.

Samarghandian S., Shoshtari M.E., Sargolzaei J., Hossinimoghadam H, & Farahzad J.A. (2014). Anti-tumor activity of safranal against neuroblastoma cells. Pharmacognosy Magazine, 10(Suppl 2), S419-S424.

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Culturing Breast Cancer and Lung Fibroblast Cells

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The human breast adenocarcinoma cell line (MCF-7) and the human fetal lung fibroblast cell line (MRC-5) were obtained from Pasteur Institute (Tehran, Iran). The cells were grown either in 96-well tissue (TC) plate (NUNC, Wiesbaden, Germany) or in 25-cm² TC flasks (NUNC, Wiesbaden, Germany), cells were cultured in CO₂ incubator MCO-17AI (Sanyo Electric Co., Ltd, Japan) at 37°C in 95% humidified atmosphere enriched by 5% CO₂ and subcultured every 3 to 4 days. The malignant (MCF-7) and nonmalignant cells (MRC-5) were cultured in DMEM supplemented with 10% (v/v) fetal bovine serum (Gibco–Invitrogen), 100 U/ml of penicillin (Gibco–Invitrogen), and 100 μg/ml streptomycin (Gibco–Invitrogen).

Samarghandian S., Hadjzadeh M.A., Afshari J.T, & Hosseini M. (2014). Antiproliferative activity and induction of apoptotic by ethanolic extract of Alpinia galanga rhizhome in human breast carcinoma cell line. BMC Complementary and Alternative Medicine, 14, 192.

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