Pgm 1

Manufactured by Santa Cruz Biotechnology

The PGM-1 is a laboratory equipment designed for the purification of biological samples. It utilizes a proprietary gel filtration technology to separate and isolate target molecules based on their size and molecular weight. The PGM-1 is a versatile tool suitable for a wide range of applications in the field of biochemistry and molecular biology.

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Lab products found in correlation

2 protocols using pgm 1

Western Blot Analysis of Cardiomyocytes

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Western or immunoblotting was performed as reported previously [7] (link). Briefly, after indicated treatments, cardiomyocytes were washed and lysed. Protein concentration was determined by a bicinchoninic acid-based assay using a commercially available kit (Thermo Fisher Scientific), and 40 µg of protein was loaded onto 10 or 12% SDS-PAGE gel for electrophoresis. Proteins were transferred to polyvinylidene fluoride (PVDF) membrane, which was blocked with 5% milk and probed with primary antibody against the protein of interest, then with anti-rabbit or anti-mouse horseradish peroxidase conjugated secondary antibody (#7074, Cell Signaling Technology) in a standard manner. Primary antibodies used for western blotting include polyclonal antibody against actin (#4968), Cyt c (#4272), ATF-2 (#9226), and CoxIV (#4844) from Cell Signaling Technology; SOD-2 (#sc-137254), PGM-1 (#sc-50656), phosphorylated p38 (#sc-17852-R), and p38β (#sc-6187-R) from Santa Cruz Biotechnology. The membrane was then incubated with chemiluminescence reagents using a commercially available ECL Plus Western Blotting Detection System (Amersham Biosciences) and exposed to film. The band intensity on radiographs was determined by densitometry and NIH ImageJ.

Liu H., Yanamandala M., Lee T.C, & Kim J.K. (2014). Mitochondrial p38β and Manganese Superoxide Dismutase Interaction Mediated by Estrogen in Cardiomyocytes. PLoS ONE, 9(1), e85272.

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Western Blotting for Glycolytic Enzymes

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Western blotting was performed according to the standard procedure to analyze the expression of G6Pase (1:100; Santa Cruz Biotechnology, #sc25840), HK I (1:100, Santa Cruz Biotechnology, #sc46695), HK II (1:100; Santa Cruz Biotechnology, #sc271459), GPI (1:100; Santa Cruz Biotechnology, #sc374091), PFK1 (1:100; Santa Cruz Biotechnology, #sc377346), FBP1 (1:100; Santa Cruz Biotechnology, #sc271241), ALDO (1:100; Santa Cruz Biotechnology, #sc166918), TPI (1:100, Santa Cruz Biotechnology, #sc166785), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:100; Santa Cruz Biotechnology, #sc47724), PGK1 (1:100; Santa Cruz Biotechnology, #sc130335), PGM1 (1:100; Santa Cruz Biotechnology, #sc373796), PKM (1:100; Santa Cruz Biotechnology, #sc365684), PC-1 (1:100; Santa Cruz Biotechnology, #sc393419), p-c-fos (1:1000; #5348, CST), c-fos (1:1000, #4384, CST), p-ERK (1:1000; #4370, CST), ERK (1:1000; #4695, CST), and β-actin (1:1000; #4970, CST).

Zhou W.J., Yang H.L., Mei J., Chang K.K., Lu H., Lai Z.Z., Shi J.W., Wang X.H., Wu K., Zhang T., Wang J., Sun J.S., Ye J.F., Li D.J., Zhao J.Y., Jin L.P, & Li M.Q. (2022). Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2+ macrophage differentiation. Science Advances, 8(8), eabj2488.

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