The GC-MS analysis was carried out for the fraction P5 at The South Indian Textile Research Association (SITRA), Coimbatore. The analysis was performed with a Thermo GC-Trace Ultra Ver: 5.0, Thermo MS DSQ II in DB 35 – MS Capillary standard non-polar column, dimensions: 30 m (L) x 0.25 mm (ID) x 0.25 µm film thickness. The carrier gas used was Helium and the flow rate was 1 ml/min. 1 µl of the sample was injected to the column. The initial temperature for the program was 70°C raised to 260°C at 6°C/min, total time was 37.50 min. Scan mass range was 50–650 m/z. The spectrum of the unknown compound was analysed using an online database for anti-cancer molecules (Cancer Resource Database – http://data-analysis.charite.de/care/ ).
Thermo gc trace ultra ver 5
Manufactured by Thermo Fisher Scientific
The Thermo GC-Trace Ultra Ver: 5.0 is a gas chromatography system designed for efficient and accurate analysis of various chemical compounds. It features advanced hardware and software components to provide reliable and consistent performance.
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4 protocols using thermo gc trace ultra ver 5
1
GC-MS Analysis of Anti-Cancer Compounds
2
Phytochemical Profiling of Methanolic Extract
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The phytochemical investigation of methanolic extract was performed on a GC-MS equipment (Thermo Scientific Co.) Thermo GC-TRACE ultra ver.: 5.0, Thermo MS DSQ II. Experimental conditions of GC-MS system were as follows: TR 5-MS capillary standard non-polar column, dimension: 30Mts, ID: 0.25 mm, Film thickness: 0.25μm. Flow rate of mobile phase (carrier gas: He) was set at 1.0 ml/min. In the gas chromatography part, temperature programme (oven temperature) was 40°C raised to 250°C at 5°C/min and injection volume was 1 μl. Samples dissolved in chloroform were run fully at a range of 50-650 m/z and the results were compared by using Wiley Spectral library search programme.
3
GC-MS Analysis of Chemical Constituents
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The GC-MS analysis was carried out using the instrument Thermo GC-Trace Ultra Ver: 5.0, Thermo MS DSQ II with Column: DB 35-MS Capillary Standard Non-Polar Column possessing Dimension: 30 Mts, ID: 0.25 mm, Film: 0.25 μm. The initial temperature of the instrument was set to 110 °C and maintained for 2 min. At the end of this period, the oven temperature was raised up to 260 °C, at the rate of an increase of 6 °C/min, and maintained for 9 min. The temperature of injection port was ensured as 250 °C and the flow rate of Helium as 1 ml/min. An injection volume of 1 µl of sample is considered in the analysis. The ionization voltage was maintained to be 70 eV and samples were injected in split mode in the ratio 10:1. Mass Spectral scan range was set at 45-450 (MHz).
The chemical constituents were identified by GC-MS. The fragmentation patterns obtained from mass spectra were compared and analysed with spectrometer database using National Institute of Standards and Technology Mass Spectral database (NIST-MS). From the relative peak area of each component in the chromatogram the percentage of each component was calculated.
The chemical constituents were identified by GC-MS. The fragmentation patterns obtained from mass spectra were compared and analysed with spectrometer database using National Institute of Standards and Technology Mass Spectral database (NIST-MS). From the relative peak area of each component in the chromatogram the percentage of each component was calculated.
4
Phytocompound Identification via GC-MS
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The effective crude extract was analyzed by GC-MS for identification of active phytocompounds. GC-MS analysis was carried out in South Indian Textile Research Association (SITRA), Coimbatore. Thermo GC-Trace Ultra Ver: 5.0, Thermo MS DSQ II equipment with diamention 30 Mts, ID: 0.25 mm, FILM: 0.25 μm was used. Helium was used as a carrier gas at flow rate 0f 1.0ml/min. The oven temperature was 70°C raised to 260°C at 6°C/min. The spectra pertaining to each RT values were further characterized using mass-spectral analysis. CAS library reference was used to elucidate the structure of compounds.
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