Cellular mda assay kit

This assay was performed according to the instructions of a cellular MDA assay kit (Nanjing Jiancheng Institute of Biological Engineering, A003-4-1). Ovarian tissues were homogenized manually with a glass homogenizer to prepare suspensions for the assay tubes (a blank tube, a standard tube, and an assay tube). The standard tube was filled with standard sample and assay working solution, and the assay tube was filled with the sample to be tested and assay working solution. They were mixed with a vortex mixer assay and placed in a water bath at 95°C for 40 min, centrifuged at 4000 rpm/min for 10 min, and placed in an enzyme marker at 530 nm to determine the OD value of each well.

DL-homocysteine (Abcam, USA); Ferrostatin-1 (Abcam, USA); BODIPY ™ 581/591 C11 (Thermo Fisher, USA); Cellular MDA Assay Kit (Jiancheng, Nanjing, China); GSH Assay Kit (Jiancheng, Nanjing, China); BCA Protein Quantification Kit (Kaiji Biological, China); GPX4 antibody (Wuhan Sanying, China); SLC7A11 antibody (Abcam, USA); GAPDH antibody (Wuhan Sanying, China); HRP-Goat anti-mouse antibody (Wuhan Sanying, China); Light microscope (Nikon, Japan) 5% CO2 constant temperature incubator (SHELL, USA); Flow cytometer (FC-500) (BD, USA); multi-functional microplate reader (Thermo Fisher, USA); BIO-RAD electrophoresis apparatus and gel imager (VDS) (BIO-RAD, USA).