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Elisa max deluxe set human for ifn γ

Manufactured by BioLegend

The ELISA Max Deluxe Set Human for IFN-γ is a laboratory equipment product designed for the quantitative measurement of human interferon-gamma (IFN-γ) in various sample types.

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2 protocols using elisa max deluxe set human for ifn γ

1

Quantifying IFN-γ Secretion in CD4+ Lymphocytes

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Supernatants from the previous assay were recovered following 5 days of coculture with CD4+ lymphocytes and stored at −80°C for further analysis of IFN-γ secretion. The quantification was performed by sandwich ELISA using ELISA Max Deluxe Set Human for IFN-γ (Cat # 430104, BioLegend) kit and following the manufacturer’s instructions. Absorbance was measured by Tecan M200 spectrofluorometer at 450 and 570 nm.
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2

Quantifying IFN-γ Secretion

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Supernatants from the previous assays were recovered following 24 h of infection and treatment and following an additional 5 days of coculture with CD4+ lymphocytes and stored at -80 °C for posterior analysis of interferon-γ (IFN-γ) secretion. The quantification was performed by Sandwich Enzyme-Linked Immunosorbent Assay using ELISA Max Deluxe Set Human for IFN-γ (Cat # 430104, Biolegend) kits and following the manufacturer’s instructions. Absorbance was measured by Tecan M200 spectrofluorometer at 570 nm.
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