MH-S cells were incubated with 50 mg/mL of BMP-IOH-NPs for various incubation periods and fixed with 4% PFA/ 2.5% glutaraldehyde in 0.1 M PBS pH 7.3. The cells were then fixed in culture dishes with 1% OsO4 (Science Services, Germany) in 0.1 M PBS and embedded in Epon (Serva, Germany) after dehydration with ethanol and en bloc staining with 1.5% uranyl acetate (Merck)/ 1.5% tungstophosphoric acid (Merck, Germany) in 70% ethanol. Ultrathin sections of cultured cells were cut parallel to the substrate using an UC7 Ultramicrotome (Leica, Germany) and stained with an aqueous solution of 4% uranyl acetate followed by lead citrate 27 (link). Sections were analysed with a LEO EM912 Omega (Zeiss, Germany) and digital micrographs were obtained with an on-axis 2048×2048 CCD camera (TRS).
Leo em912 omega
Manufactured by Zeiss
Sourced in Germany
The LEO EM912 Omega is an electron microscope designed for high-resolution imaging and analysis of a wide range of materials. It features a LaB6 electron source, advanced optics, and a modular design that allows for customization to meet specific research needs.
Automatically generated - may contain errors
Lab products found in correlation
Tungstophosphoric acid, by Merck Group (3 mentions)
Uranyl acetate, by Merck Group (3 mentions)
Uc7 ultramicrotome, by Leica (2 mentions)
Uranyless, by Science Services (1 mentions)
Exwave had, by Sony (1 mentions)
Uranyl acetate, by Leica (1 mentions)
Ultracut s ultramicrotome, by Leica (1 mentions)
4 protocols using leo em912 omega
1
Ultrastructural Analysis of MH-S Cells Treated with BMP-IOH-NPs
2
Ultrastructural Analysis of iPSC-CM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IPSC-CM were fixed with 4% formaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.3 and prepared for electron microscopy essentially as described [47 (link)]. In brief, the adherent cultures were postfixed on the culture dish with 1% OsO4 (Science Services, Munich, Germany) in 0.1 M phosphate buffer and embedded in Epon (Serva, Heidelberg, Germany) after dehydration with ethanol and en bloc staining with 1.5% uranyl acetate (Merck, Darmstadt, Germany) and 1.5% tungstophosphoric acid (Merck, Darmstadt, Germany) in 70% ethanol. Ultrathin sections of cultured cells were cut parallel to the substrate using an UC7 Ultramicrotome (Leica, Vienna, Austria) and stained with UranyLess® (Science Services, Munich, Germany). Sections were analyzed with an LEO EM912 Omega (Zeiss, Oberkochen, Germany) and digital micrographs were obtained with an on-axis 2048 × 2048-CCD camera (TRS, Moorenweis, Germany).
3
Characterization of SWCNT Nanostructures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy was conducted on a Zeiss Leo EM912 Omega with an acceleration voltage of 80 kV. Specimens were prepared through dip casting of either lacey carbon/copper or lacey carbon supported ultrathin carbon/copper grids into a suspension of SWCNT/1 or SWCNT/SDBS. The sample grids were dried at 80 °C for 5 h prior to microscopy.
Atomic force microscopy tapping mode images were recorded on a Solver Pro scanning probe microscope (NT-MDT) equipped with a Sony Exwave HAD camera optical zoom (6.5). The carbon nanotube material was spread on oxidized silicon wafers with a 200 nm thermally grown oxide layer through spin coating (100 rps).
Atomic force microscopy tapping mode images were recorded on a Solver Pro scanning probe microscope (NT-MDT) equipped with a Sony Exwave HAD camera optical zoom (6.5). The carbon nanotube material was spread on oxidized silicon wafers with a 200 nm thermally grown oxide layer through spin coating (100 rps).
4
Cell Fixation and Embedding for TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SK8/18-2 cells were fixed by adding to the culture medium 2× concentrated fixative (8% formaldehyde, 5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.3). After removal of the supernatant after 5 min, the fixation was continued with 4% formaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.3 for at least 4 h. The cells were then postfixed in 1% OsO4 (Science Services, Munich, Germany) in 0.1 M phosphate buffer and embedded in Epon (Serva, Heidelberg, Germany) after dehydration with ethanol and en bloc staining with 1.5% uranyl acetate (Merck, Darmstadt, Germany) and 1.5% tungstophosphoric acid (Merck) in 70% ethanol. Ultrathin sections of cultured cells were cut parallel to the substrate using an Ultracut S Ultramicrotome (Leica, Vienna, Austria) and stained with an aqueous solution of 4% uranyl acetate followed by lead citrate (16 (link)). Sections were analyzed with a LEO EM912 Omega (Zeiss, Oberkochen, Germany), and digital micrographs were obtained with an on-axis 2048 × 2048-CCD camera (TRS, Moorenweis, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!