Percp anti cd45.1 a20

Manufactured by BioLegend

The (PerCP)-anti-CD45.1 (A20) is a fluorescently-labeled antibody that binds to the CD45.1 antigen. CD45.1 is a common leukocyte antigen expressed on the surface of various immune cells. The PerCP fluorophore is used to label the antibody, enabling detection and analysis of CD45.1-positive cells through flow cytometry.

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Lab products found in correlation

Anti cd25 apc pc61, by Thermo Fisher Scientific (1 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Pe anti cd25 pc61.5, by Thermo Fisher Scientific (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Annexin 5 fitc pi, by BD (1 mentions)

Close spelling variants of this product

Anti-CD45 (228 citations) CD45-PerCP (44 citations) CD45.1 (A20, (29 citations) Anti-CD45-PerCP (24 citations) Anti-CD45.1 (A20, (10 citations) Anti-CD45.1-PerCP/Cy5.5 (A20), (2 citations)

2 protocols using percp anti cd45.1 a20

Multicolor Flow Cytometry Analysis

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Immunofluorescence staining of peripheral blood, spleen and lymph node single-cell suspensions was performed using the following monoclonal antibodies: allophycociannin (APC)-CD4 (GK1.5) and phycoerythrin (PE) anti-CD25 (PC61.5), purchased from eBioscience, and fluorescein isothiocyanate (FITC)-anti-CD8 (YTS 156.7.7) and peridinin chlorophyll protein (PerCP)-anti-CD45.1 (A20), purchased from BioLegend. For neuropilin-1 staining, PE anti-Nrp-1/CD304 (3E12, Biolegend) was used in conjunction with APC anti-CD25 (PC61.5, eBioscience). Cell membrane permeabilization and Foxp3 intracellular staining, using either Alexa488- or biotin-conjugated anti-Foxp3 monoclonal antibody (FJK-16 s, eBioscience) in conjunction with PECy7-streptavidin, were performed according to the instructions contained in eBioscience commercial kit. Stained cells were acquired with AccuriC6 (AccuriCytometers) flow cytometer and analyzed with FlowJo v8.7 software (Treestar).

do Canto F.B., Campos S.M., Granato A., da Silva R.F., de Paiva L.S., Nóbrega A., Bellio M, & Fucs R. (2016). Enlarged colitogenic T cell population paradoxically supports colitis prevention through the B-lymphocyte-dependent peripheral generation of CD4+Foxp3+ Treg cells. Scientific Reports, 6, 28573.

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Multiparametric Flow Cytometry Analysis

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FITC-anti-CD3 (clone 145-2C11), IA/IE (2G9), PE-CD69 (H1·2F3), CD11c (HL3), PerCP-Cy5.5-Ly6G (1A8), APC-anti-CD4 (RM4-5), CD11b (M1/70) (BD Pharmingen), Alexa Fluor 488-Ly6C (HK1.4), PE-Ly6G (1A8) (eBioscience), PerCP-anti-CD45.1 (A20), and PerCP-anti-CD45.2 antibody (104) (BioLegend) were used for cell-surface staining. Apoptosis was assessed by staining for FITC-annexin V/PI, and FITC-anti-active caspase 3 (C92-605) according to the manufacturer’s protocols (BD Pharmingen). Stained cells were analyzed on a FACSCalibur flow cytometer (Becton Dickinson, Tokyo).

Miyagawa F., Tagaya Y., Ozato K., Horie K, & Asada H. (2020). Inflammatory monocyte-derived dendritic cells mediate autoimmunity in murine model of systemic lupus erythematosus. Journal of Translational Autoimmunity, 3, 100060.

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