Thermo trace gc ultra dsq 2

The GC/MS analysis was performed using a Thermo Trace GC Ultra/DSQ II chromatograph (Thermo Fisher Scientific, Waltham, MA, USA).
Operating parameters of the gas chromatography were adapted as follows: column—non-polar stationary phase Rxi–1 ms (length 60 m, internal diameter 0.25 mm, film thickness 0.25 μm, Restek Corp., Bellefonte, PA, USA), injector temperature: 280 °C, FID detector temperature: 300 °C, carrier gas—helium 5.0, constant pressure 300 kPa and split ratio 1:50, oven temperature program was 50 °C for 3 min, 50 to 300 °C at 4°/min, 300 °C for 10 min. Mass spectrometry parameters: ion source temperature 200 °C, ionization energy 70 eV. The quantity of the individual components was achieved using a flame-ionization detector connected through the MS-FID splitter (SGE Analytical Science, Ringwood, Melbourne, VIC, Australia). Databases from the NIST Library (RRID:SCR_014680), Wiley 8th edition, and the Adams 4th edition were used. All samples were injected three times.

Essential oils were isolated through hydro-distillation from 5.00 g dry plant weighted portions using a Clevenger-type apparatus. The makeup of volatile compounds in the oil composition was determined using Thermo Trace GC Ultra/DSQ II (Thermo Fisher Scientific, Waltham, MA, USA). Operating parameters of the gas chromatography were set up as follows: column—non-polar stationary phase Rxi–1 ms (length 60 m, internal diameter 0.25 mm, film thickness 0.25 μm, Restek Corp., Bellefonte, PA, USA), injector temperature: 280 °C, FID detector temperature: 300 °C, carrier gas—helium, constant pressure 300 kPa and split ratio 1:100, oven temperature program was 50 to 300 °C at 4°/min. Mass spectrometry parameters: ion source temperature 200 °C, ionization energy 70 eV. The quantity of the individual components was expressed as a percentage of the essential oil and was achieved using flame-ionization detector connected through the MS-FID splitter (SGE Analytical Science, Ringwood, Melbourne, VIC, Australia). Databases from the NIST Library, Wiley 8th edition and the Adams 4th edition were used. All samples were tested in triplicates.

Essential oils were isolated through hydrodistillation from 5.00-g dry plant-weighted portions using a Clevenger-type apparatus. The makeup of volatile compounds in the oil composition was determined using Thermo Trace GC Ultra/DSQ II (Thermo Fisher Scientific, Waltham, MA, USA). Operating parameters of the gas chromatography were set up as follows: column: nonpolar stationary phase Rxi⁻¹ ms (length 60 m, internal diameter 0.25 mm and, film thickness 0.25 μm; Restek Corp., Bellefonte, PA, USA); injector temperature: 280 °C; FID detector temperature: 300 °C; carrier gas: helium, constant pressure 300 kPa and split ratio 1:100; oven temperature program was 50–300 °C at 4°/min. Mass spectrometry parameters: ion source temperature 200 °C and ionization energy 70 eV. The quantity of the individual components was expressed as a percentage of the essential oil and was achieved using a flame ionization detector connected through the MS-FID splitter (SGE Analytical Science, Ringwood, Melbourne, VIC, Australia). Databases from the NIST Library, Wiley 8th edition and the Adams 4th edition were used.