Szx10 fluorescence microscope

We purchased dexamethasone sodium phosphate (Dex) injection solution (H42020019, ≥97%) from Tianjin Jinyao Group, Hubei Tianyao Pharmaceutical Industry Co. Ltd.; Res from Sigma (USA, CAS no.: 501-36-0; ≥99%, 1002179582); alizarin red S (100375) and dimethyl sulfoxide (DMSO, 196055) from MP Biomedicals; low-melting-point agarose from Life Technologies (16520-100); methylcellulose from Sigma (1001777023); Tween-20 from Guangdong Guanghua Sci-Tech Co. Ltd. (9005-64-5); anhydrous ethanol from Shanghai Sangon Biotech (ET0737); and methanol from Tianjin Damao Chemical Reagent Factory (67-56-1).
We used a SZX10 fluorescence microscope from Olympus (Germany), a Leica fluorescence microscope-imaging system (M2057A) from Leica (Germany), the Image Pro Plus 6.0 professional image analysis software from Media Cybernetics (USA), and a thermostat incubator.

We took zebrafish with 1 × tricaine (0.016 mg/mL) and images were captured using an OLYMPUS SZX10 fluorescence microscope coupled with a DP71 and U-RFL-T camera (OLYMPUS, Tokyo, Japan) at 1 dpi. The embryos were then placed in a 96-well plate, with 280 μL per well containing one zebrafish embryo. The drugs were dissolved in E3/PTU buffer and 1% DMSO as control. At 2 dpi, we changed the media with fresh solution and control. Two days after drug treatment (3 dpi), the images of the same embryo were captured again. The tumor cells in embryos were observed, and the migration and proliferation ability of the injected cells were recorded and analyzed using ImageJ software (National Institutes of Health (NIH), Bethesda, MD, USA).