The phenolic profile composition of the canned lychee was identified using the reversed-phase HPLC-DAD method described by Zhang et al.14 (link) on an Agilent 1260 HPLC system (Agilent Technologies Deutschland GmbH, Oberhaching, Germany) equipped with an Agilent 1260 series DAD detector. HPLC analysis was performed using a YMC-Pack ODS-A column (250 × 4.6 mm; I.D., 5 μm; YMC Karasuma-Gojo Bldg, Japan) at 30 °C. The sample injection volume was 20 μL, and the mobile phase flow rate was 1.0 mL min−1. The mobile phase included solvent A (water/acetic acid, 996 : 4, v/v) and solvent B (acetonitrile). The gradient was programed as follows: 0–40 min, 5–25% B; 40–45 min, 25–35% B; and 45–50 min, 35–50% B. Chromatographic data were detected at 280 nm. Quercetin-3-rutinose-7-rhamnoside, gallic acid, (−)-gallocatechin, procyanidin B2, vanillic acid, syringic acid, procyanidin A2, ferulic acid, and rutin were used as standards. The details of the calibration and correlation coefficients of the 9 phenolics are supplied in Table S1.† Values are expressed as μg per g of lychee pulp. The data are reported as the mean ± SD of three replications obtained from three samples.
Agilent 1260 series dad detector
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 1260 series DAD (Diode Array Detector) is a high-performance liquid chromatography (HPLC) detector. It is designed to provide precise and reliable detection of a wide range of analytes in various liquid chromatography applications. The 1260 series DAD detector utilizes a diode array technology to simultaneously monitor multiple wavelengths, enabling comprehensive spectral analysis of the samples.
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Lab products found in correlation
Nicolet 6700, by Thermo Fisher Scientific (6 mentions)
Rudolph autopo 5, by Rudolph Research Analytical (3 mentions)
Agilent 1260 series, by Agilent Technologies (3 mentions)
Brighttime chirascan, by Applied Photophysics (3 mentions)
Avance 3 500 spectrometer, by Bruker (2 mentions)
Agilent 6520 q tof mass spectrometer, by Agilent Technologies (2 mentions)
Agilent 1260 hplc system, by Agilent Technologies (1 mentions)
Pack ods a column, by YMC (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Thermo scientific nicolet 6700, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Avance 3 500, by Bruker (1 mentions)
Avance 3 600, by Bruker (1 mentions)
4 protocols using agilent 1260 series dad detector
1
Quantifying Phenolic Profiles in Canned Lychee
2
Isolation and Purification of Natural Products
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Column chromatography (CC): silica gel H (10–40 μm) and silica gel (200–300 mesh) (Marine Chemical Factory, Qingdao, P. R. China); MCI gel CHP–20P: (Daiso, Co., Japan) and RP-C18 gel (40–63 μm; Daiso, Co., Japan) were used for column chromatography; Sephadex LH-20 (Pharmacia Fine Chemicals, Piscataway, NJ, USA). TLC: silica gel plates, visualization by spraying with 10% H2SO4 in EtOH and Dragendorff's reagent. Semi-preparative HPLC: Agilent 1260 series (Agilent Technologies, US) with a Zorbax SB-C18 (5 μM, 9.4 mm × 25 cm) column. NMR Spectra: Bruker Avance III-500 spectrometer (Bruker, Switzerland). MS: Agilent MSD-Trap-XCT (for ESI) and Agilent-6520 Q-TOF mass spectrometer (for HR-ESI). IR: Thermo Scientific Nicolet 6700 (Thermo Scientific, US). UV spectra: Agilent 1260 series DAD detector (Agilent Technologies, US). CD spectrum: Brighttime Chirascan (Applied Photophysics Ltd, UK). Optical rotation: Rudolph Autopo V (Rudolph Research Analytical, Hackettstown, NJ).
3
Column Chromatography and Spectroscopic Analysis
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Column chromatography (CC): silica gel H (10–40 μm) and silica gel (200–300 mesh) (Marine Chemical Factory, Qingdao, P. R. China). MCI gel CHP-20P: (Daiso, Co., Japan) and RP-C18 gel (40–63 μm; Daiso, Co., Japan) were used for column chromatography. Sephadex LH-20 (Pharmacia Fine Chemicals, Piscataway, NJ, USA); TLC: silica gel plates, visualization by spraying with 10% H2SO4 in EtOH and Dragendorff's reagent. Semi-preparative HPLC: Agilent 1260 series (Agilent Technologies, US) with a Zorbax SB-C18 (5 μM, 9.4 mm × 25 cm) column. NMR spectra: Bruker Avance III-500 spectrometer (Bruker, Switzerland). MS: Agilent MSD-Trap-XCT (for ESI) and Agilent-6520 Q-TOF mass spectrometer (for HR-ESI). IR: Thermo Scientific Nicolet 6700 (Thermo Scientific, US). UV spectra: Agilent 1260 series DAD detector (Agilent Technologies, US). CD spectrum: Brighttime Chirascan (Applied Photophysics Ltd, UK). Optical rotation: Rudolph Autopo V (Rudolph Research Analytical, Hackettstown, NJ).
4
Chromatographic Purification and Analytical Methods
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Column chromatography (CC): silica gel H (10–40 μm) and silica gel (200–300 mesh) (Marine Chemical Factory, Qingdao, P. R. China); Sephadex LH-20 (Pharmacia Fine Chemicals, Piscataway, NJ, USA); RP-C18 gel (40−63 μm; Daiso, Co., Japan). TLC: silica gel plates (Yantai Jiang You Silicone Development Co., Yantai, P. R. China), visualization by spraying with 10% H2SO4 in EtOH. HPLC: Agilent 1260 series (Agilent Technologies, US) with a Zorbax SB-C18 (5 μm, 9.4 × 150 mm) column. NMR: Bruker Avance III-500 and Avance III-600 spectrometers (Bruker, Switzerland). MS: Agilent MSD-Trap-XCT (for ESI) and Agilent-6520 Q-TOF mass spectrometers (for HR-ESI). Melting point: X-4B digital display micro-melting apparatus (Shanghai Jingsong Instrument, Shanghai, P. R. China). Optical rotation: Rudolph Autopo V (Rudolph Research Analytical, Hackettstown, NJ). UV: Agilent 1260 series DAD detector (Agilent Technologies, US). CD: Brighttime Chirascan (Applied Photophysics Ltd, UK). IR: MTTiCZIBPBHhf-iF1qfc/" target="_blank">Thermo Scientific Nicolet 6700 (Thermo Scientific, USA). RAW 264.7 cells and 293T cells: ATCC (American type culture collection). Dulbecco’s modified Eagle’s medium and fetal bovine serum: Gibco Invitrogen (Carlsbad, CA, USA). LPS Griess reagent and MTT: Sigma-Aldrich (St. Louis, MO, USA).
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