Paraformaldehyde bl539a

The target gene fragments were amplified by PCR and were inserted into pcDNA3.0 (Invitrogen) to construct eukaryotic expression vectors. The target sequence of BEND5 shRNA was 5'-GCAAATACGTCGTCCTATT-3'. The human breast cancer cell lines MDA-MB-231 and ZR75-1, and human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection. Anti-BEND5 (20931-1-AP), anti-HEY2 (10597-1-AP), anti-E-cadherin (20874-1-AP), anti-N-cadherin (22018-1-AP), anti-Vimentin (10366-1-AP), and anti-MAML1 (55493-1-AP) were purchased from Proteintech. Anti-HES1 (ab71559) was purchased from Abcam. Anti-BEND5 (NBP2-26211) was from Novus Biologicals. Anti-RBPJ (sc-271128), anti-Notch1 (sc-373891), and anti-β-actin (sc-47778HRP) were purchased from Santa Cruz Biotechnology. Anti-FLAG (A8592), anti-FLAG M2 agarose (A2220), anti-c-MYC gel (E6654), and anti-c-MYC-peroxidase (A5598) were from Sigma-Aldrich. Dulbecco's modified Eagle's medium (DMEM, 12491-015) was purchased from Gibco. VigoFect reagent (T001) was purchased from Vigorous Biotechnology. CCK8 reagent (CK04) was purchased from Dojindo. Paraformaldehyde (BL539A) was purchased from Biosharp.

Rabbit hairs were collected from German Angora rabbit warren (Gansu, China). Urea and Sodium bisulfite were purchased from Damao chemical reagent Co. Ltd. (Tianjin, China). Sodium dodecyl sulfate (SDS) was purchased from Usolf Co. Ltd. (Shenzhen, China). Acrylamide, N, N′-methylene-bisacrylamide, ammonium persulfate, pyrogallol, glycerin, methanol acetic acid, bromophenol blue and glycine were purchased from FUCHEN chemical reagent Co. Ltd. (Tianjin, China). Coomassie brilliant blue R-250 was purchased from Sigma-Aldrich (Shanghai, China). Tetramethylethylenediamine (TEMED), β-Mercaptoethanol and Tris-HCl Buffer were purchased from MACKLIN (Shanghai, China). BSA (0332-100G) was purchased from VWR (Westchester, PA, USA). Paraformaldehyde (BL539A) was purchased from BioSharp (Hefei, China). TritonX-100 (90002-93-1) was purchased from Biotopped (Beijing, China). Cell Counting Kit-8 (CCK-8 C0038) and DAPI (C1002) were obtained from Beyotime Institute of Biotechnology (Shanghai, China). Primary antibody (β-actin AF7018) and fluorescent secondary antibody (FITC S0006 Goat Anti-Rabbit IgG (H+L) Fluor594-conjugated) were purchased from Affinity Biosciences (Golden, CO, USA).