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2 protocols using hygromycin b

1

Chemical Compound Procurement for Biological Assays

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SDS, sorbitol, DTT, NaCl, and LiCl were purchased from Wako Pure Chemical Industries (Osaka, Japan). Fluconazole, voriconazole, hygromycin B, caffeine, and monensin sodium salt were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Nourseothricin was purchased from Jena Bioscience (Dortmund, Germany). Congo red was purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA). CaCl2 was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Tunicamycin was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Brefeldin A (BFA) was purchased from Funakoshi Co., Ltd. (Tokyo, Japan). Human serum [(from male AB clotted whole blood), USA origin, sterile-filtered] (Product ID: H6914) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Antifungal and Stress Resistance Assay Protocol

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Nourseothricin was purchased from Jena Bioscience (Dortmund, Germany). Cefotaxime sodium, D-glucose, agar, NaCl, KCl, sorbitol, H2O2, DTT, MMS, and AMPH-B were purchased from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). Hygromycin B, caffeine, FLCZ, VRCZ, and N-phenylthiourea were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Congo Red and menadione were purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA). Hipolypeptone was purchased from Nihon Pharmaceutical Co., Ltd. (Tokyo, Japan). Tunicamycin (TM) was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Sodium dodecyl sulfate (SDS) was purchased from Nippon Gene Co., Ltd. (Tokyo, Japan). Tween 80 was purchased from MP Biomedicals LLC (Santa Ana, MO, USA).
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