Log In Sign up
The largest database of trusted experimental protocols

Methanol ch3oh

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in China, United States

Methanol (CH3OH) is a colorless, volatile liquid chemical compound. It is a simple alcohol with the molecular formula CH3OH. Methanol serves as a versatile solvent and can be used in various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Lc ms grade water, by Thermo Fisher Scientific (2 mentions) Dna probe, by Sangon (1 mentions) Dexamethasone (dex), by Maclin Biochemical Technology (1 mentions) Acetonitrile c2h3n, by Thermo Fisher Scientific (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions) 37 fame standard mix, by Merck Group (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphatidylinositol, by Avanti Polar Lipids (1 mentions) 1 nonadecanoyl 2 hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phospho 10 rac glycerol, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions) N heptadecanoyl d erythro sphingosine, by Avanti Polar Lipids (1 mentions) N heptadecanoyl d erythro sphingosylphosphorylcholine, by Avanti Polar Lipids (1 mentions) Oleylamine, by Merck Group (1 mentions) Copper 2 acetylacetonate cu acac 2, by Merck Group (1 mentions) Dicyandiamide, by Merck Group (1 mentions) Urea nh2conh2, by Merck Group (1 mentions) Nitric acid 65 hno3, by Merck Group (1 mentions) Dichloromethane, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Methanol (2986 citations) CH3OH (8 citations)

10 protocols using methanol ch3oh

1

Aptamer-Guided Gold Nanoparticle Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA probes (aptamer sequence: 5′-ACA CGA CGA GGG ACG AGG AGT ACT TGC CAA CGA TAA CGT CGT TGG ATC TGT CTG TGC CC-3′) were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China). HAuCl4·H2O was purchased from Beijing Chemical Reagent Company (Beijing, China). Dexamethasone was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Trisodium citrate dihydrate was obtained from Yongsheng Fine Chemicals Company (Tianjin, China). Methanol (CH3OH) and Acetonitrile (C2H3N) were obtained from Fisher Scientific Co., Ltd. (Shanghai, China). Ethyl acetate(C4H8O2) was purchased from Tianjin Beilian Fine Chemicals Development Co., Ltd. (Tianjin, China). Milk and glucosamine samples were obtained at the local market. All experiments were repeated three times, and the standard deviation (error bars) on three sets of measurements were calculated by Origin software.
Qin Y., Bubiajiaer H., Yao J, & Zhang M. (2022). Based on Unmodified Aptamer-Gold Nanoparticles Colorimetric Detection of Dexamethasone in Food. Biosensors, 12(4), 242.
+ Open protocol
+ Expand
2

Fatty Acid Methyl Ester Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chloroform (CHCl3), sulfuric acid (H2SO4), and anhydrous sodium sulphate (Na2SO4) were obtained from Merck (Germany), and methanol (CH3OH) and sodium hydroxide (NaOH) were procured from Fisher Chemicals (UK). The 37 FAME standard mix was purchased from Supelco (USA). All reagents, solvents, and standards were of analytical grade.
Abd Rahman M.R., Hassan Z., Hassan M.S., Hashim R., Wong L.S., Leong W.Y., Syd Jaafar S.H, & Salvamani S. (2023). Enhanced Goat Milk MUFA Quality via Date Pit Supplementation: A Time-Based Pattern Recognition Analysis Utilizing Agricultural Waste Byproduct. International Journal of Food Science, 2023, 1797017.
+ Open protocol
+ Expand
3

Analytical Reagents and Lipid Standards for HPLC-based Lipidomics

Check if the same lab product or an alternative is used in the 5 most similar protocols
High-performance liquid chromatography (HPLC)-grade dichloromethane (CH2Cl2), absolute ethanol 96% (CH3CH2OH), and methanol (CH3OH) were obtained from Fisher Scientific Ltd. (Loughborough, UK). Milli-Q purified water (Synergy1, Millipore Corporation, Billerica, MA, USA) was used in all experiments. The 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS●+) was purchased from Fluka (Buchs, Switzerland) and the α,α-diphenyl-β-pic-rylhydrazyl radical (DPPH●) from Aldrich (Milwaukee, WI, USA). All the remaining reagents were obtained from primary commercial sources. The lipid internal standards used in the lipidome analyses were acquired from Avanti Polar Lipids, Inc. (Alabaster, AL, USA): 1,2-dimyristoyl-sn-glycero-3-phosphate (dMPA), 1,2-dimyristoyl-sn-glycero-3-phospho-(10-rac-glycerol) (dMPG), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylserine (dMPS), 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC), N-heptadecanoyl-D-erythro-sphingosine (Cer), and 1′,3′-bis-[1-2-di-tetradecanoyl-sn-glycero-3-phospho]-sn-glycerol (CL), and N-heptadecanoyl-D-erythro-sphingosylphosphorylcholine (SM).
Maurício T., Couto D., Lopes D., Conde T., Pais R., Batista J., Melo T., Pinho M., Moreira A.S., Trovão M., Barros A., Cardoso H., Silva J., Domingues P, & Domingues M.R. (2023). Differences and Similarities in Lipid Composition, Nutritional Value, and Bioactive Potential of Four Edible Chlorella vulgaris Strains. Foods, 12(8), 1625.
+ Open protocol
+ Expand
4

Copper-Bipyridine Complex Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following chemicals were purchased and used as-received without further purification: Copper(II) chloride dihydrate (CuCl2·2H2O, ACS grade, Sigma Aldrich), 2,2,-bipyridine (C10H8N2, reagent grade, Sigma Aldrich), oxalic acid (HO2CCO2H, reagent grade, Alfa Aesar), urea (NH2CONH2, ACS grade, Sigma Aldrich), dicyandiamide (NH2C( = NH)NHCN, ACS grade, Sigma Aldrich), copper(II) acetylacetonate (Cu(acac)2, ACS reagent, Sigma Aldrich), oleylamine (CH3(CH2)7CH = CH(CH2)8NH2, ≥ 98%, Sigma Aldrich), ethanol (C2H5OH, HPLC grade, Fisher Scientific), methanol (CH3OH, HPLC grade, Fisher Scientific), deionized water (18.2 MΩ) was collected from an ELGA PURELAB flex apparatus.
Xie P., Ding J., Yao Z., Pu T., Zhang P., Huang Z., Wang C., Zhang J., Zecher-Freeman N., Zong H., Yuan D., Deng S., Shahbazian-Yassar R, & Wang C. (2022). Oxo dicopper anchored on carbon nitride for selective oxidation of methane. Nature Communications, 13, 1375.
+ Open protocol
+ Expand
5

Synthesis and Photolysis of XDPAdeCage

Check if the same lab product or an alternative is used in the 5 most similar protocols
The XDPAdeCage chelator (see Scheme 1) was synthesized as described previously.42 ,43 (link) The substrate strand (/5FAM/-ACTCACTATrAGGAAGAGATGGACGTG-/3BHQ/), and enzyme strand (CACGTCCATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT-/3Dab/) of the 8–17 DNAzyme were synthesized by the IDT. 4-(2-Hydroxyethyl)-1-piperizineethanesulfonic acid (HEPES), tris(hydroxymethyl)aminomethane (Tris), sodium chloride (NaCl, 99.8%), zinc chloride (ZnCl2,99.8%), dichloromethane (CH2Cl2), methanol (CH3OH), bovine newborn calf serum (BNCS), 4-(2-pyridylazo)resorcinol (PAR), and Triton X-114 were purchased from Fisher Scientific. Trace element serum L-2 RUO was purchased from Sero. Nitric acid (HNO3 65%) was purchased from Merck. The Zn2+ stock solution was prepared from ZnCl2 using Millipore water. CH3OH was routinely used as a co-solvent for preparing the stock solutions of the chelator. Photolysis was carried at 25 °C in a 1.0 cm quartz cuvette illuminated by 3 W UV light-emitting diode (LED) purchased from LED Engin (365 nm, 200 mW) powered by a 700 Ma LuxDriveFlexBox using a variable DC source set at 12 V DC.
Xing S., Lin Y., Cai L., Basa P.N., Shigemoto A.K., Zheng C., Zhang F., Burdette S.C, & Lu Y. (2021). Detection and Quantification of Tightly Bound Zn2+ in Blood Serum Using a Photocaged Chelator and a DNAzyme Fluorescent Sensor. Analytical chemistry, 93(14), 5856-5861.
+ Open protocol
+ Expand
6

Spectrophotometric Determination of p-Nitrophenol

Check if the same lab product or an alternative is used in the 5 most similar protocols

p-nitrophenol (C6H5NO3, Cas: 100-02-7, Macklin Biochemical Technology Co., Ltd., Shanghai, China, the physical and chemical properties are shown in Table 1); hydrochloric acid (HCl, Beijing Chemical Plant Co., Ltd., Beijing, China); methanol (CH3OH, high-performance liquid chromatography grade, Thermo Fisher Scientific, Waltham, USA); sodium bicarbonate (NaHCO3, Sigma Aldrich (Shanghai) Trading Co., Ltd., Shanghai, China); ascorbic acid (C6H8O6, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); calcium chloride (CaCl2), manganese sulfate (MnSO4·H2O), potassium nitrate (KNO3), ferric chloride (FeCl3·6H2O), magnesium sulfate (MgSO4·7H2O), and potassium chloride (KCl) were purchased from Tianjin Bodi Chemical Co., Ltd., Tianjin, China. All the reagents involved in this article were of analytical grade or higher. All solutions were prepared with ultrapure water from a pure water treatment process (≥18 MΩ∙cm, Milli-Q Advantage A10, Millibo (Shanghai) Trading Co., Ltd., Shanghai, China).
Xia H., Zhang W., Yang Z., Dai Z, & Yang Y. (2021). Spectrophotometric Determination of p-Nitrophenol under ENP Interference. Journal of Analytical Methods in Chemistry, 2021, 6682722.
+ Open protocol
+ Expand
7

Synthesis of Simulated Marine Aquaculture

Check if the same lab product or an alternative is used in the 5 most similar protocols
FLU (99%) was purchased from Shanghai Yuanye Biological Company. Sodium persulfate (Na2S2O8), sodium chloride (NaCl), sodium sulfate (Na2SO4), sodium bromide (NaBr) and sodium bicarbonate (NaHCO3) were analytical-reagent grade purchased from Chengdu Jinshan Chemical Reagent Company. HPLC grade formic acid, acetonitrile and methanol (CH3OH) were purchased from Thermo Fisher Scientific. Ultrapure water obtained from the Millipore Milli-Q Ultrapure Gradient system was used to prepare all solutions. The essential anions including Cl− (19 g L−1), Br− (65 mg L−1), HCO3− (152 mg L−1) and SO42− (2.74 g L−1) were added into ultrapure water to prepare the synthetic seawater according the components of actual seawater. The synthetic marine aquaculture was prepared by synthetic seawater and ultrapure water in a ratio of 2 : 1. Therefore, the concentrations of the essential anions were approximately one-third of that in synthetic seawater. The pH of all samples was adjusted to 7 with phosphate buffer.
Zhang Y., Huang K., Zhu Y., Chen X., Wei M, & Yu K. (2022). Kinetics and mechanisms of flumequine degradation by sulfate radical based AOP in different water samples containing inorganic anions. RSC Advances, 12(16), 10088-10096.
+ Open protocol
+ Expand
8

Quantification of Glyphosate and Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glyphosate
(99%), AMPA (99%), and glufosinate-ammonium (99%) were obtained from
Chemservice (West Chester, PA). Primary stock solutions containing
1 μg/μL concentrations for all chemicals (corrected for
purity) were prepared in deionized (DI) water. A 100 μg/L working
solution of glyphosate, glufosinate, and AMPA was prepared and stored
at 4 °C. For sample fortification and calibration standards,
several combined solutions of all target compounds ranging from 0.5
to 10 μg/L were created and employed as spike solutions. Isotopically
labeled standards, 1,2-13C215N glyphosate
(99%) and 13C15N AMPA (99%), were obtained from
Cambridge Isotope Laboratories (Andover, MA). The internal standard
solution was made in DI water with a 50 μg/L concentration.
LC–MS grade water, EDTA, acetonitrile (ACN), sodium tetraborate,
FMOC-Cl, methanol (CH3OH), and phosphoric acid (H3PO4) were obtained from Fischer Scientific (Hampton, NH).
A 0.1% phosphoric acid (v/v) in ACN/DI water (70:30) was used for
rinsing the autosampler before and after injection.
Martin P.J., He K., Blaney L, & Hobbs S.R. (2023). Advanced Liquid Chromatography with Tandem Mass Spectrometry Method for Quantifying Glyphosate, Glufosinate, and Aminomethylphosphonic Acid Using Pre-Column Derivatization. ACS Es&t Water, 3(8), 2407-2414.
+ Open protocol
+ Expand
9

Analysis of Tea Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals, reagents, and organic solvents of the highest grade available were purchased from Merck (St. Louis, MO, USA) unless otherwise stated. LC-MS grade water, methanol (CH3OH), and acetonitrile (ACN) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The tea powder was bought in a local supermarket.
Montone C.M., Aita S.E., Arnoldi A., Capriotti A.L., Cavaliere C., Cerrato A., Lammi C., Piovesana S., Ranaldi G, & Laganà A. (2021). Characterization of the Trans-Epithelial Transport of Green Tea (C. sinensis) Catechin Extracts with In Vitro Inhibitory Effect against the SARS-CoV-2 Papain-like Protease Activity. Molecules, 26(21), 6744.
+ Open protocol
+ Expand
10

Analytical Methods for Reagent Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The reactants used in this study include hydrogen peroxide (H2O2, 6% solution, ACS GR) from VWR Chemicals (Radnor, PA, USA), chloridric acid (HCl, 36.5–38.0%, ACS GR), Folin–Ciocaleau’s Reagent (1.8–2.2 mol/L), sodium carbonate (NaCO3, ACS GR), and sodium hydroxide (NaOH, beads) from PanReac (Barcelona, Spain), sulfuric acid (H2SO4, 95.0–98.0%, ACS GR) from Fluka (Charlotte, NC, USA), TPTZ (2,4,6-Tris (2-pyridil)-s-triazine, ≥99.0%), and iron (III) chloride hexahydrate (FeCl3·6H2O, ≥98%) from Sigma-Aldrich (St. Louis, MO, USA).
The solvents, used as received without further purification, include acetone ((CH3)2CO, ≥99.0%), ethanol (C2H5OH, 99.9%) from VWR Chemicals, and methanol (CH3OH, ≥95%) from Thermo Scientific (Waltham, MA, USA).
Milli-Q water (Barnstead™ Pacific TII Water, Thermo Scientific) throughout this study.
Pirozzi A., Olivieri F., Castaldo R., Gentile G, & Donsì F. (2023). Cellulose Isolation from Tomato Pomace: Part II—Integrating High-Pressure Homogenization in a Cascade Hydrolysis Process for the Recovery of Nanostructured Cellulose and Bioactive Molecules. Foods, 12(17), 3221.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!