Polyphenols

Manufactured by Merck Group

Sourced in United States

Polyphenols are a class of chemical compounds found naturally in various plant-based foods. They are antioxidant-rich substances that can be extracted and used in laboratory settings for research and analysis purposes. Polyphenols play a role in the color, taste, and health benefits of many plant-based foods and beverages.

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Polyphenol standards (6 citations) Tea polyphenols (2 citations)

3 protocols using polyphenols

Antioxidant Activity Evaluation Protocol

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2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), butylated hydroxytoluene (BHT), sodium azide, guanidine hydrochloride, tween-40, 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin Ciocalteau reagent, β-carotene, linoleic acid, glucose, 2,4- dinitrophenylhydrazine (DNPH), 5,5’-Dithiobis-(2-Nitrobenzoic Acid) (DTNB), ammonium molybdate, and polyphenols standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrogen peroxide (H₂O₂) was purchased from Fluka (Basel, Switzerland). Aluminum chloride (AlCl₃), sodium carbonate (Na₂CO₃), ferric chloride (FeCl₃), potassium persulphate (K₂S₂O₈), potassium ferricyanide (K₃Fe(CN)₆), sulfuric acid, and all solvents were obtained from Merck Life Science (Darmstadt, Germany). Ascorbic acid and trichloracetic acid (TCA) were obtained from Scharlau (Barcelona, Spain).

Guenaou I., Nait Irahal I., Errami A., Lahlou F.A., Hmimid F, & Bourhim N. (2021). Bioactive Compounds from Ephedra fragilis: Extraction Optimization, Chemical Characterization, Antioxidant and AntiGlycation Activities. Molecules, 26(19), 5998.

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Antioxidant Polyphenol Evaluation

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All chemicals and reagents were obtained from Sigma Aldrich (USA) if no indications were given. All antibodies were purchase from Santa Cruz if no indications were given. RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco (Life Technologies, USA). Penicillin-streptomycin solution was obtained from Hyclone (Thermo Scientific, USA). 2′, 7′-dichlorofluorescein diacetate (DCFH-DA) was obtained from Molecular Probes (USA). Polyphenols were obtained from Sigma-Aldrich (USA).

Wang H., Li D., Hu Z., Zhao S., Zheng Z, & Li W. (2016). Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats. Molecules and Cells, 39(6), 508-513.

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Antimicrobial Evaluation of Phytochemicals

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The following reagents and chemicals were used in this study: polyphenols, 1,8-cineole, and anhydrous sodium sulfate from Sigma-Aldrich; Nutritional Agar (NA); Sabouraud Dextrose Agar with chloramphenicol (SDA); Műller-Hinton Broth (MHB); Sabouraud Dextrose Broth (SDB); ciprofloxacin (CPH 5 μg/disc); fluconazole (FLC 25 μg/disc).

Stefanova G., Girova T., Gochev V., Stoyanova M., Petkova Z., Stoyanova A, & Zheljazkov V.D. (2020). Comparative study on the chemical composition of laurel (Laurus nobilis L.) leaves from Greece and Georgia and the antibacterial activity of their essential oil. Heliyon, 6(12), e05491.

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