Cleaved parp

Manufactured by Novus Biologicals

Sourced in United Kingdom

Cleaved PARP is a lab equipment product that detects the presence of cleaved PARP protein, which is a marker of apoptosis or programmed cell death. It is used in scientific research to analyze cellular processes.

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Lab products found in correlation

Cleaved caspase 9, by Novus Biologicals (1 mentions) Ecl system, by CWBIO (1 mentions) Tom20, by Abcam (1 mentions) Affinipure goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Nucleolin, by Merck Group (1 mentions) Bcl 2, by Novus Biologicals (1 mentions) Aldh1a1, by Novus Biologicals (1 mentions) Iκb α, by Novus Biologicals (1 mentions) Bcl2 associated x (bax), by Novus Biologicals (1 mentions) Ecl western blotting detection kit, by Geneflow (1 mentions) Anti α tubulin, by Merck Group (1 mentions)

2 protocols using cleaved parp

Characterization of Mitochondrial Proteins

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Western blot analysis was performed as detailed previously (40 (link),45 (link),46 (link)). Twenty micrograms of total cell proteins obtained from various cell lines were denatured and loaded on sodium dodecyl sulfate polyacrylamide gels. The gels were electroblotted onto a polyvinylidene difluoride membrane for hybridization. The antibodies obtained from different companies were as follows: Abcam [TOM20 (ab56783), ND1 (ab74257), ND5 (ab92624), CO1 (ab695), CO2 (ab110258), ND6 (ab81212), Total OXPHOS Human WB Antibody Cocktail (ab110411) and CYTC (ab13575)], Cell Signaling Technology [Cleaved Caspase 3 (#9664), Cleaved Caspase 9 (#7237), Cleaved PARP (#5625), SOD1 (4266T), SOD2 (13141T) and Catalase (12980T)], Novus [ND4 (NBP2-47365)] and Proteintech [CYTB (55090-1-AP), ATP8 (26723-1-AP), CO3 (55082-1-AP), Afg3l2 (14631-1-AP), Clpp (15698-1-AP), Lonp1 (66043-1-AP) and GAPDH (10494-1-AP)]. Peroxidase AffiniPure goat anti-mouse IgG and goat anti-rabbit IgG (Jackson) were used as a secondary antibody and protein signals were detected using the ECL system (CWBIO). Quantification of density in each band was performed as detailed previously (40 (link),45 (link),46 (link)).

Xiao Y., Wang M., He Q., Xu L., Zhang Q., Meng F., Jia Z., Zhang F., Wang H, & Guan M.X. (2020). Asymmetrical effects of deafness-associated mitochondrial DNA 7516delA mutation on the processing of RNAs in the H-strand and L-strand polycistronic transcripts. Nucleic Acids Research, 48(19), 11113-11129.

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Protein Expression Quantification Protocol

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The protein expression levels were determined by staining with primary antibodies and relevant HRP conjugated secondary (1:5000, Armersham, Buckinghamshire, UK) antibodies. The primary antibodies (Santa Cruz, CA, USA: Bcl2, Bax, p65, IκBα, nucleolin, ALDH1A1, 1A3, 3A1, 2 and cleaved PARP; Novus Bio, Cambridge, UK: HIF2; Abcam, Cambridge, UK: phosphorylated p65_S276; Cell Signaling, Herts, UK: AKT, phosphorylated AKT, Sox2, Oct4, JNK, phosphorylated JNK, cJun, phosphorylated cJun, phosphorylated p38, ERK) were diluted at 1:1000 in 5% fat-free milk-TBST. Anti-α-tubulin (1:8000, Sigma) and nucleolin were used as a loading control. The signal was detected using an ECL Western blotting detection kit (GeneFlow, Staffordshire, UK).

Liu P., Wang Z., Brown S., Kannappan V., Tawari P.E., Jiang W., Irache J.M., Tang J.Z., Britland S., Armesilla A.L., Darling J.L., Tang X, & Wang W. (2014). Liposome encapsulated Disulfiram inhibits NFκB pathway and targets breast cancer stem cells in vitro and in vivo. Oncotarget, 5(17), 7471-7485.

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