Mjf r21

Manufactured by Abcam

The MJF-R21 is a laboratory equipment product. It is a multi-function instrument designed for various applications in a research or testing laboratory setting. The core function of the MJF-R21 is to perform tasks related to sample preparation, analysis, or measurement, but the specific details of its capabilities are not available.

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Lab products found in correlation

Mouse monoclonal anti gapdh, by Proteintech (1 mentions) Nb300 109, by Novus Biologicals (1 mentions) Mjf r20, by Abcam (1 mentions) Mouse monoclonal anti β tubulin, by Merck Group (1 mentions) Mjf r21 22 5, by Abcam (1 mentions) Mouse monoclonal anti gfp, by Merck Group (1 mentions) Goat anti mouse igg hrp, by Jackson ImmunoResearch (1 mentions) Hspa8, by Cell Signaling Technology (1 mentions) Irdye 800cw donkey anti rabbit, by LI COR (1 mentions) Mini protean tgx stain free gel, by Bio-Rad (1 mentions) Mjff2, by Abcam (1 mentions) Donkey anti mouse irdye 680lt, by LI COR (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Immobilon fl membrane, by Merck Group (1 mentions) Anti tyrosine hydroxylase, by Merck Group (1 mentions) Gsk2578215a, by Bio-Techne (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Lrrk2 in 1, by Merck Group (1 mentions) Mli 2, by Bio-Techne (1 mentions)

Close spelling variants of this product

MJF-R21-22–5

3 protocols using mjf r21

Antibody Profiling of LRRK2 Modifications

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Primary antibodies used include: mouse monoclonal anti-GFP (clones 7.1 and 13.1, Sigma), mouse monoclonal anti-LRRK2 (clone N241A/34, NeuroMabs), rabbit monoclonal anti-LRRK2 (clone MJFF2/c41-2, Abcam), mouse monoclonal anti-FLAG and anti-FLAG-HRP (clone M2, Sigma), rabbit monoclonal anti-pSer910-LRRK2 [clone UDD1 15 (3 (link)), Abcam], rabbit monoclonal anti-pSer935-LRRK2 [clone UDD2 10 (12 (link)), Abcam], rabbit monoclonal anti-pSer1292-LRRK2 (clone MJFR-19-7-8, Abcam), mouse monoclonal anti-β-tubulin (TUB 2.1, Sigma), rabbit polyclonal anti-dynamin-1 (PA1-660, Thermo Fisher Scientific), rabbit polyclonal anti-tyrosine hydroxylase (NB300-109, Novus Biologicals), rabbit monoclonal anti-phospho-Thr73-RAB10 (MJF-R21 or MJF-R21-22–5, Abcam), anti-phospho-Thr72-RAB8A (MJF-R20, Abcam), rabbit monoclonal anti-RAB10 (D36C4, Cell Signaling Technology), mouse monoclonal anti-human adenovirus 5, hexon capsid protein (clone 9C12, Developmental Studies Hybridoma Bank), mouse monoclonal anti-GAPDH (clone 1E6D9, Protein Tech). For bright-field microscopy, biotinylated goat anti-mouse and anti-rabbit secondary antibodies (Vector Laboratories) were employed. For Western blots, light chain-specific mouse anti-rabbit IgG-HRP and goat anti-mouse IgG-HRP (Jackson Immunoresearch) secondary antibodies were used.

Nguyen A.P., Tsika E., Kelly K., Levine N., Chen X., West A.B., Boularand S., Barneoud P, & Moore D.J. (2020). Dopaminergic neurodegeneration induced by Parkinson's disease-linked G2019S LRRK2 is dependent on kinase and GTPase activity. Proceedings of the National Academy of Sciences of the United States of America, 117(29), 17296-17307.

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LRRK2 and Rab10 Phosphorylation Assay

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Protein lysates were electrophoresed on a 7.5% Mini-Protean TGX stain- free gel (BioRad) and then transferred onto Immobilon-FL membranes (Millipore) and blocked with Odyssey Blocking Buffer (LiCOR). Membranes were cut in half below the high-molecular weight markers. Primary antibodies included mouse anti-LRRK2 clone N241A/34 (1: 2000, Antibodies, Inc.), rabbit anti-LRRK2 antibody MJFF2 (1:2000, Abcam), Ser(P)-935 (1: 2000, Abcam), Ser(P)-1292 (1:2000, Abcam) HSPA8 (1:5000, Cell Signaling), phospho-T73-Rab10 antibody (1:1000, MJF-R21, Abcam), and total Rab10 (1:1000, clone D36C4, Cell Signaling). Secondary antibodies were IRdye 680LT donkey anti-mouse and IRdye 800CW donkey anti-rabbit (both 1:10000, LiCOR). Membranes were scanned using a LiCOR CLx with 685-nm and 785-nm lasers and band intensities were quantified using the LiCOR-Odyssey system.

Kelly K., Wang S., Boddu R., Liu Z., Moukha-Chafiq O., Augelli-Szafran C, & West A.B. (2018). The G2019S Mutation in LRRK2 Imparts Resiliency to Kinase Inhibition. Experimental neurology, 309, 1-13.

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Antibodies and Mouse Model for LRRK2

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Antibodies for LRRK2 [MJFF2 (C41-2)] (# ab133474), phospho-S935-LRRK2 [UDD2 10 ( 12)] (#ab133450), phospho-T73-Rab10 [MJF-R21] (# ab230261), Rab10 [MJF-R23] (# ab237703), and RPL10a (# ab55544) were from Abcam. Anti-tyrosine hydroxylase was from Millipore (#AB1542). Antibodies for eIF2B5(sc-28854), eIF2α (sc-11386), phospho-eIF2α-S52 (sc-101670), eIF4G3 (sc-100732), and Ndufs3 (sc-292169) were from Santa Cruz Biotechnology. Anti-Rab8 (#R66320) and Rab4 (#R68520) were from BD Transduction Laboratories. Anti-4E-BP1 (# 9452), eEF2 (#2332) and phospho-eEF2(T57) (#2331) were from Cell Signaling Technology and anti-actin (# A3853) was from Sigma-Aldrich. LRRK2-IN1 was from Merck Millipore (# 438193), GSK-2578215A (#4629) and MLi-2 (# 5756) were from Tocris Bioscience. Miglyol 812N was from Cemer Oleo, GmbH, and Co, KG (Germany). Patient cells were from the National Institute of Neurological Disorders and Stroke (NINDS) repository at the Coriell Institute for Medical Research and the Cell Line and DNA Biobank from Patients Affected by Genetic Diseases, Telethon Network of Genetic Biobanks (TNGB) (project no. GTB12001). 23 C57BL/6-Lrrk2 tm1.1Mjff /J mice developed by Michael J. Fox Foundation 24 were obtained from The Jackson Laboratory (Stock no. 016121).

Deshpande P., Flinkman D., Hong Y., Goltseva E., Siino V., Sun L., Peltonen S., Elo L.L., Kaasinen V., James P, & Coffey E.T. (2020). Protein synthesis is suppressed in sporadic and familial Parkinson's disease by LRRK2. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(11).

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