Rabbit anti cd8

Formalin fixed and paraffin‐embedded (FFPE) tissue sections were stained with hematoxylin and eosin (H&E) for evaluation by light microscopy. To identify microscopic oviductal lesions, each oviduct and ovary were serially sectioned in their entirety. Alternate sections were either stained with H&E or retained for immunohistochemical (IHC) staining, performed according to standard methods as previously described (9 (link),24 (link)). The primary antibodies used were: rabbit anti-MYC, anti-PTEN, anti-FOXP3 (Cell Signaling Technology, Danvers, MA), rabbit anti‐PAX8 (Proteintech, Chicago, IL, USA), and rabbit anti-CD8 (Abcam, Cambridge, UK).

IHC staining was used to determine expression levels of HHLA2, B7x, CD4, CD8, CD11b, Ki67, PCNA, and Caspase 3 in pancreatic tissues from NET patients and Men1 KO and WT mice. Purified mouse anti-HHLA2 (Zhao et al. 2013 (link)) and anti-B7x antibodies (obtained from Dr. Zang’s laboratory, Einstein; 1:50 dilution)(Zang et al., 2007 (link)), rabbit anti-CD4 (1:1000 dilution; Abcam, MA, USA), rabbit anti-CD8 (1:200 dilution; Abcam, MA, USA), mouse-anti-HIF-1α (1:200 dilution; Abcam, MA, USA), rabbit anti-Ki67 (1:100 dilution; Abcam, MA, USA), rabbit anti-CD11B (1:400 dilution; Novus Biologicals, CO, USA), rabbit anti-proliferating cell nuclear antigen (PCNA) (1:200 dilution; Santa Cruz, CA, USA), and rabbit anti-Caspase 3 (1:200 dilution; BD Pharmingen, CA, USA) antibodies were used and incubated overnight at 4°C. A HEP/DAB (ABC) detection IHC kit (Abcam, MA, USA) was used for IHC analysis. An IHC staining without primary antibody was used as a negative control and a recommended tissue for positive expression of the antibody was used as a positive control.