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Mbs448092

Manufactured by MyBioSource
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The MBS448092 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor and can accommodate various types of sample tubes. The centrifuge provides controlled speed and time settings to separate components within liquid samples.

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Lab products found in correlation

A21447, by Thermo Fisher Scientific (2 mentions) Gfp 1020, by Merck Group (2 mentions) A5228, by Merck Group (1 mentions) Ab5320, by Merck Group (1 mentions) Ab9260, by Merck Group (1 mentions) Axiolab microscope, by Zeiss (1 mentions) Axio imager fluorescence microscope, by Zeiss (1 mentions) Dia 310, by Dianova (1 mentions) Sc 338, by Santa Cruz Biotechnology (1 mentions) A21206, by Jackson ImmunoResearch (1 mentions) Sc 9059, by Santa Cruz Biotechnology (1 mentions) Sc 374522, by Santa Cruz Biotechnology (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) A21432, by Thermo Fisher Scientific (1 mentions) A11055, by Thermo Fisher Scientific (1 mentions) Ab10135, by Abcam (1 mentions) Ab9361, by Abcam (1 mentions) Clone 1a4, by Agilent Technologies (1 mentions) A31570, by Jackson ImmunoResearch (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions)

5 protocols using mbs448092

1

Visualizing mRNA and Protein Expression

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mRNA transcripts were visualized using the RNAscope Multiplex Fluorescent Reagent Kit v2. After completing the RNAscope protocol, immunostaining was performed to visualize the expressed tdTomato protein. Slides were blocked and stained overnight using a goat anti-tdTomato antibody (MyBioSource MBS448092) at a 1:100 dilution, then incubated with a donkey anti-goat antibody (Thermo Fisher Scientific A-21447) at a 1:250 dilution and counterstained with DAPI.
Radmand A., Kim H., Beyersdorf J., Dobrowolski C.N., Zenhausern R., Paunovska K., Huayamares S.G., Hua X., Han K., Loughrey D., Hatit M.Z., Del Cid A., Ni H., Shajii A., Li A., Muralidharan A., Peck H.E., Tiegreen K.E., Jia S., Santangelo P.J, & Dahlman J.E. (2024). Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart. Proceedings of the National Academy of Sciences of the United States of America, 121(11), e2307801120.
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2

Immunohistochemical Analysis of Angiogenesis and Muscle Regeneration

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Paraffin sections were deparaffinized and rehydrated and subjected to antigen retrieval in Antigen Retrieve Solution (HK086-9K, Biogenex) at 95°C for 20 minutes. Sections were blocked with 5% horse serum for 1 hour at room temperature. Mouse tissues were incubated with anti-CD31 (1:100, catalog DIA-310, dianova), anti-tdTomato (1:100, catalog MBS448092, MyBioSource), anti–Ki-67 (1:100, catalog AB9260, MilliporeSigma), anti–NG-2 (1:100, catalog AB5320, MilliporeSigma), anti–α-SMA (1:100, catalog A5228, MilliporeSigma), and anti-eMHC (catalog F1.652, DSHB) antibodies overnight at 4°C. For Matrigel plug assay, sections were incubated with anti-CD31 antibody overnight at 4°C. Sections were stained with Alexa Fluor 488–conjugated (catalog A-21202, catalog A-11006, catalog A-11034, Thermo Fisher Scientific), 568–conjugated (catalog 11079, catalog A-11004, Thermo Fisher Scientific), and 647–conjugated (catalog 405416, BioLegend) appropriate secondary IgGs (1:500) for 1 hour at room temperature. Images were acquired with an Axio Imager fluorescence microscope (ZEISS). Images were quantified by ImageJ software (NIH). For histological study, sections were stained with H&E and imaged with an Axio Lab microscope (ZEISS).
Duan H., He Z., Lin M., Wang Y., Yang F., Mitteer R.A., Kim H.J., Yeo E., Han H., Qin L., Fan Y, & Gong Y. (2020). Plasminogen regulates mesenchymal stem cell–mediated tissue repair after ischemia through Cyr61 activation. JCI Insight, 5(15), e131376.
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3

Immunofluorescence Staining and Confocal Analysis

Cited 1 time
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Immunofluorescence staining and confocal analysis of larvae and 1-month-old fish were performed as previously described27 (link). Primary antibodies were used against GFP (to amplify the GFP-signal, 1:500; Aves Labs GFP-1020), glucagon (1:200; Sigma G6254), insulin (1:100; custom made by Cambridge Research Biochemicals), and tdTomato (1:500; MYBioSource MBS448092). For experiments in juveniles, the ins:GFP+ area was measured on a flattened projection (average intensity). For the OPP intensity measurements, all larvae were imaged using the same parameters on a confocal microscope. Quantification was performed using the Fiji parameter (mean gray value). The mean gray value was measured from 8 tp1:GFP+ cells around the islet from a single-plane for each larva using the same area as reference for each cell, and then the average of the mean gray value of the 8 cells was calculated. All images were acquired with the LAS X (v3.5.5.19976) software. The contrast was adjusted for visualization purposes in some experiments, in which case the same adjustments were made for all displayed images from the same experiment. The original, unmodified pictures were used for analysis.
Karampelias C., Watt K., Mattsson C.L., Ruiz Á.F., Rezanejad H., Mi J., Liu X., Chu L., Locasale J.W., Korbutt G.S., Rovira M., Larsson O, & Andersson O. (2022). MNK2 deficiency potentiates β-cell regeneration via translational regulation. Nature Chemical Biology, 18(9), 942-953.
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4

Quantitative Immunofluorescence Analysis of Larval and Juvenile Fish

Cited 1 time
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Immunofluorescence staining and confocal analysis of larvae and 1-month-old fish were performed as previously described27 (link). Primary antibodies were used against green fluorescent protein (GFP; to amplify the GFP signal, 1:500; Aves Labs, GFP-1020), glucagon (1:200; Sigma, G6254), insulin (1:100; custom made by Cambridge Research Biochemicals) and tdTomato (1:500; MYBioSource, MBS448092). For experiments in juveniles, the insulin:GFP+ area was measured on a flattened projection (average intensity). For the OPP intensity measurements, all larvae were imaged using the same parameters on a confocal microscope. Quantification was performed using the Fiji parameter (mean gray value). The mean gray value was measured from eight tp1:GFP+ cells around the islet from a single plane for each larva using the same area as a reference for each cell, and the average of the mean gray value of the eight cells was calculated. All images were acquired with LAS X (v3.5.5.19976) software. The contrast was adjusted for visualization purposes in some experiments, in which case the same adjustments were made for all displayed images from the same experiment. The original unmodified pictures were used for analysis.
Karampelias C., Watt K., Mattsson C.L., Ruiz Á.F., Rezanejad H., Mi J., Liu X., Chu L., Locasale J.W., Korbutt G.S., Rovira M., Larsson O, & Andersson O. (2022). MNK2 deficiency potentiates β-cell regeneration via translational regulation. Nature Chemical Biology, 18(9), 942-953.
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5

Comprehensive Immunofluorescence Staining Protocol

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The following chemical reagent and antibodies were used for immunofluorescence: Alexa Fluor 633 hydrazide (Elastin) (A30634, Invitrogen), goat anti-TDTomato (1:200, MBS448092, MyBioSource.com), rabbit anti-Connexin 43 (Cx43) (1:200, SC-9059, Santa Cruz Biotechnology), mouse anti-Smooth Muscle Actin (αSMA) (1:300, Clone 1A4, Dako), goat anti-TAGLN (1:200, ab10135, Abcam), chicken anti-β Galactosidase (1:200, ab9361, Abcam), FITC-conjugated mouse anti-CD34 (1:200, 11–0341-82, Invitrogen), mouse anti-LEF1 (1:100, SC-374522, Santa Cruz Biotechnology), rabbit anti-PDGFRA (1:100, sc-338, Santa Cruz Biotechnology), chicken anti-GFP (1:200, AB-2307313, Aveslab), rabbit anti-MEOX2 (1:200, NBP2–30647, Novus Biological). Alexa-488, 555, 594 or Alexa-647-conjugated donkey anti-goat, anti-rabbit, anti-mouse, anti-chicken secondary antibodies (all from Invitrogen; A11055, A21432, A21447, A21206, A21207, A31573, A21202, A31570, A21203, A31571, A78952), Alexa-488 or Alexa 594- conjugated donkey anti-chicken secondary antibodies (703–545-155, and 703–585-155 from Jackson ImmunoResearch).
Yin Y., Koenitzer J.R., Patra D., Dietmann S., Bayguinov P., Hagan A.S, & Ornitz D.M. (2023). Identification of a myofibroblast differentiation program during neonatal lung development. bioRxiv.
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