Migration of cells was performed using QCM 24-Well Colorimetric Migration Assay Kit (Millipore) according to the manufacturer's instructions. For the invasion assay, Cell Invasion Assay Kit (Millipore) was used. Cells (2 × 104) in 200 μl serum-free medium were added to the upper chambers and cultured for 48 h. Non-migrating or non-invading cells were removed with cotton swabs, cells that migrated or invaded to the bottom of the membrane were stained with the cell stain buffer and counted under microscope and photographed. Three independent experiments were performed for the same conditions.
Qcm 24 well colorimetric migration assay kit
Manufactured by Merck Group
The QCM 24-Well Colorimetric Migration Assay Kit is a laboratory instrument designed to measure cell migration. It utilizes a 24-well format and a colorimetric detection method to quantify the migratory behavior of cells.
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2 protocols using qcm 24 well colorimetric migration assay kit
1
Cell Migration and Invasion Assay
2
Cell Migration Assay Protocol
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Migration of cells was performed using QCM™24-Well Colorimetric Migration Assay Kit (Millipore) according to the manufacturer’s instructions. Cells (1 × 105) in 300 µl serum-free medium were added to the upper chambers and cultured for 48 h and 72 h. Non-migrating or non-invading cells were removed with cottons swabs, cells that migrated to the bottom of the membrane were stained, and counted under microscope and photographed. Three independent experiments were performed for each sample.
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