Smart apex ccd crystallography system

Column chromatography (CC) was run on silica gel (200–300 mesh, Qingdao Marine Chemical, Inc.) and Lichroprep RP-18 (40–63 μm, Merck). Semipreparative HPLC was carried out on an Agilent 1100 liquid chromatography system using a YMC-Pack 10 mm × 250 mm column (Pro C18 RS). Precoated TLC plates (200–250 μm thickness, silica gel 60 F₂₅₄, Qingdao Marine Chemical, Inc.) were used for thin-layer chromatography. 1D and 2D NMR spectra were obtained using Bruker DRX-500 and Avance III-600 MHz spectrometers (Bruker, Zűrich, Switzerland) with solvent signal as internal reference. ESIMS and HRESIMS were run with a Shimadzu LCMS-IT-TOF mass spectrometer (Shimadzu, Kyoto, Japan) or an Agilent G6230 TOF MS (Agilent Technologies, Palo Alto, USA). Infrared spectra were obtained using a Shimadzu IR-450 instrument with KBr pellets. A JASCO P-1020 digital polarimeter was applied to record optical rotations, using MeOH as solvent. X-ray diffraction was realized with a Bruker SMART APEX CCD crystallography system. Flow cytometry analysis was conducted with a PARTEC brand flow cytometer (Germany). Quantitative PCR (q-PCR) was conducted with a ProFlex™ PCR system (Thermo Fisher, Shanghai, China). SDS-PAGE was carried out with a Mini-Protean Tetra Electrophoresis System (Bio-Rad, Shanghai, China).