Quercetin (Sigma, USA) was prepared into a 200 mM stock using dimethyl sulfoxide (DMSO) and stored at −20°C. The working concentration of 200 μM Quercetin was made in a complete medium, and a range of concentrations of Quercetin (10, 20, 50, 100, 150 and 200 μM) was utilized in cell viability assay. Cisplatin (Sigma, USA) and paclitaxel (MedChemExpress, USA) were stored at −20°C, while 5-fluorouracil (Solarbio, China) and doxorubicin (Solarbio, China) were stored at 4°C. Primary antibodies for MMP2 (monoclonal rabbit anti-human antibody; 1:1000), ezrin (polyclonal rabbit anti-human; 1:1000) and GAPDH (monoclonal rabbit anti-human antibody; 1:1000) were purchased from Cell Signaling Technology, and P-Gp (monoclonal rabbit anti-human antibody; 1:1000) and METTL3 (monoclonal rabbit anti-human antibody; 1:1000) were purchased from Abcam (1:1000, USA); and the second antibody, peroxidase-conjugated goat anti-rabbit antibody, was obtained from Biosharp (1:5000, USA). Diaminobenzidine (DAB) Substrate Kit was purchased from Zhongshan Goldenbridge (China).
Diaminobenzidine dab substrate kit
Manufactured by Zhongshan Golden Bridge Biotechnology
Sourced in China
The Diaminobenzidine (DAB) Substrate Kit is a laboratory reagent used for the detection of specific proteins or enzymes in biological samples. It provides a chromogenic substrate that, when catalyzed by the target enzyme, produces a brown or black precipitate. This reaction can be visualized using light microscopy, allowing for the localization and quantification of the target analyte within the sample.
Automatically generated - may contain errors
Lab products found in correlation
Paclitaxel, by MedChemExpress (2 mentions)
Doxorubicin, by Solarbio (1 mentions)
Mettl3, by Abcam (1 mentions)
Ezrin, by Cell Signaling Technology (1 mentions)
5 fluorouracil, by Solarbio (1 mentions)
Peroxidase conjugated goat anti rabbit antibody, by Biosharp (1 mentions)
Mmp 2, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Quercetin, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Propofol, by Merck Group (1 mentions)
2 protocols using diaminobenzidine dab substrate kit
1
Quercetin Cytotoxicity Evaluation Protocol
2
Propofol Cytotoxicity Assay Protocol
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Propofol (Sigma, USA) was stored at room temperature, and two series of various concentrations of Propofol (clinically relevant concentrations: 0, 5, 10, 20, 40, and 80 μM; high concentrations: 0, 100, 200, 400, 600, and 800 μM) were applied in the cell viability assay. Paclitaxel (MedChemExpress, USA) was stored at −80°C. The diaminobenzidine (DAB) substrate kit was obtained from Zhongshan Goldenbridge (China). The primary antibodies utilized were as follows: rabbit anti‐β‐tubulin (1:2000, ABclonal, AC008, China), rabbit anti‐stathmin 1 (1:1000, Abcam, ab52630, USA) and rabbit anti‐GAPDH (1:2000, CST, #5174, USA). The secondary antibody was a horseradish peroxidase‐conjugated goat anti‐rabbit antibody (1:2000, Biosharp, USA).
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