Trisodium citrate

Cur (Mw = 368.38, purity ≥98%), sodium hydroxide, and trisodium citrate were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was provided by Sigma-Aldrich Co. (St. Louis, MO). The dialysis bags were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM), penicillin–streptomycin, and fetal bovine serum (FBS) was purchased from Gibco BRL (Gaithersburg, MD). ROS detection kit was obtained from Bio Lebo Technology Co., Ltd. (Beijing, China). Hoechst 33342 was purchased from Beyotime Biotechnology (Nanjing, China). The water used in the experiments was deionized. Other reagents were of analytic grade. MDA-MB-231 and 4T1 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). Female Balb/c mice (4–5 weeks old) were obtained from the Laboratory Animal Center of Nantong University.

Tenuazonic acid and other common mycotoxin standards, bovine serum albumin (BSA) and goat anti-mouse IgG antibody (GAMA) were obtained from Sigma-Aldrich (MO, United States). Trisodium citrate, HAuCl₄ 4H₂O and hydroquinone were purchased from Macklin (Shanghai, China). Nitrocellulose (NC) membrane was provided by Jieyi BioTech. (Shanghai, China). Anti-TA antibody reagent (anti-TA McAb 6D5) was prepared in our laboratory according to our previous work (Cai et al., 2021 (link)). All the other reagents were analytical reagent grade. Ultraviolet-visible spectrum (UV-VIS) was tested by spectrophotometer (PerkinElmer, Waltham, MA, United States). Tecnai G2 F30 transmission electron microscope (TEM) (FEI, Hillsboro, OR, United States) was used to scan the diameter and shape of the prepared nanoparticles. The UHPLC-MS/MS was performed by Waters ACQUITY UPLC-XEVO TQ-S MS with ACQUITY UPLC BEH C18 (Waters, Milford, MA, United States).