CAL27 and UM-SCC-74A cells were transduced with Dharmacon siGENOME SMART pool siRNA for Myoferlin. In brief, tumor cells were cultured in 60 mm dishes and transfected with 100 nM of siRNA SMART pool. Cells transfected with scrambled siRNA were used as control. After 16–18 hours of incubation, cells were washed with PBS and further incubated in fresh medium. Seventy two hours post transfection, cells were used for experiments. For stable knockdown, we used two shRNAs; MISSION® Myoferlin shRNA Lentiviral transduction particles from Sigma-Aldrich and Myoferlin shRNA (m) Lentiviral particles from Santa Cruz Biotechnology. MISSION® pLKO.1-puro carrying scrambled shRNA was used as a control. The lentiviral constructs were transduced in the cells in 96 well plate using polybrene to the final concentration of 4μg/ml. The transfected cells were selected with puromycin treatment and myoferlin knockdown was confirmed by Western blotting.
Mission myoferlin shrna lentiviral transduction particles
Manufactured by Merck Group
MISSION® Myoferlin shRNA Lentiviral transduction particles are a tool used for the delivery of short hairpin RNA (shRNA) sequences targeting the myoferlin gene into cells. The product consists of lentiviral particles that can transduce a variety of cell types and induce knockdown of myoferlin expression.
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Lab products found in correlation
2 protocols using mission myoferlin shrna lentiviral transduction particles
1
Myoferlin Knockdown in Oral Cancer Cells
2
Myoferlin Knockdown in Oral Cancer Cells
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CAL27 and UM-SCC-74A cells were transduced with Dharmacon siGENOME SMART pool siRNA for Myoferlin. In brief, tumor cells were cultured in 60 mm dishes and transfected with 100 nM of siRNA SMART pool. Cells transfected with scrambled siRNA were used as control. After 16–18 hours of incubation, cells were washed with PBS and further incubated in fresh medium. Seventy two hours post transfection, cells were used for experiments. For stable knockdown, we used two shRNAs; MISSION® Myoferlin shRNA Lentiviral transduction particles from Sigma-Aldrich and Myoferlin shRNA (m) Lentiviral particles from Santa Cruz Biotechnology. MISSION® pLKO.1-puro carrying scrambled shRNA was used as a control. The lentiviral constructs were transduced in the cells in 96 well plate using polybrene to the final concentration of 4μg/ml. The transfected cells were selected with puromycin treatment and myoferlin knockdown was confirmed by Western blotting.
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