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Hibernate a hb a

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Hibernate-A (Hb-A) is a laboratory equipment product designed for maintaining low temperature environments. It serves the core function of providing controlled temperature conditions for storage and preservation of samples or materials.

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Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (2 mentions) L cysteine, by Merck Group (2 mentions) Papain, by Merck Group (2 mentions) Neurobasal a, by Thermo Fisher Scientific (2 mentions) Neurobasal plus nb, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Hibernate A (26 citations)

2 protocols using hibernate a hb a

1

Isolation and Culture of Primary Mouse Hippocampal Neurons

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Hippocampi from P0 or P1 mouse pups (CD1 strain) were dissected in ice-cold Hibernate-A (Hb-A) (Gibco) supplemented with 5mM MgCl2, and then dissociated using papain (Sigma-Aldrich) pre-activated with L-cysteine (Sigma-Aldrich) in Hb-A/MgCl2. Tissue was further triturated, and the neuronal suspension was filtered through a 70μm cell strainer and centrifuged. Neurons were plated at a density of ~1.0–1.3×105 neurons per 12mm PDL-coated coverslip (Neuvitro) in Neurobasal-A (Nb-A) or Neurobasal-plus (Nb+) media (Gibco) supplemented with 2% B27+ (Gibco), 0.16% Glutamax (Gibco) and ~0.5–1% FBS (Hyclone). Approximately half the media was replaced every 3 days with Nb-A media, 2% B27+, and 0.16% Glutamax. Neurons were maintained for 12–18 days in vitro (DIV) before experiments.
Aow J., Huang T.R., Goh Y.T., Sun A.X., Thinakaran G, & Koo E.H. (2023). Evidence for a clathrin-independent endocytic pathway for APP internalization in the neuronal somatodendritic compartment. Cell reports, 42(7), 112774.
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2

Isolation and Culture of Primary Mouse Hippocampal Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hippocampi from P0 or P1 mouse pups (CD1 strain) were dissected in ice-cold Hibernate-A (Hb-A) (Gibco) supplemented with 5mM MgCl2, and then dissociated using papain (Sigma-Aldrich) pre-activated with L-cysteine (Sigma-Aldrich) in Hb-A/MgCl2. Tissue was further triturated, and the neuronal suspension was filtered through a 70μm cell strainer and centrifuged. Neurons were plated at a density of ~1.0–1.3×105 neurons per 12mm PDL-coated coverslip (Neuvitro) in Neurobasal-A (Nb-A) or Neurobasal-plus (Nb+) media (Gibco) supplemented with 2% B27+ (Gibco), 0.16% Glutamax (Gibco) and ~0.5–1% FBS (Hyclone). Approximately half the media was replaced every 3 days with Nb-A media, 2% B27+, and 0.16% Glutamax. Neurons were maintained for 12–18 days in vitro (DIV) before experiments.
Borgese F., Sardet C., Cappadoro M., Pouyssegur J, & Motais R. (1992). Cloning and expression of a cAMP-activated Na+/H+ exchanger: evidence that the cytoplasmic domain mediates hormonal regulation.. Proceedings of the National Academy of Sciences of the United States of America, 89(15), 6765-6769.
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