Human GC cell lines (AGS, SGC-7901, MGC-803, MKN45, BGC-823 and KATO III) were obtained from the Chinese Academy of Medical Sciences. Purified Tregs were co-cultured with SGC7901 cells in RPMI-1640 supplemented with penicillin (100 U/mL), streptomycin (100 mg/mL), L-glutamine (2 mM), beta-hydroxy ethyl alcohol (55 mM, Gibco, Australia), sodium pyruvate (10 mM, Sigma, USA) and 10% FBS (Gibco, Australia). Next, SGC7901 cells were respectively, treated with 1 × 104 purified Tregs or various concentrations of recombinant TGF-β1 protein (R&D Systems, 240-B-002 USA), with or without human TGF-β1 neutralizing antibody (AF-246-NA, R&D Systems, USA) or the TGF-β/ALK5/Smad2 inhibitor (SB431542, Abcam, USA). Lgr5 and β-catenin expression were examined by qRT-PCR and western blot.
Recombinant tgf β1 protein
Manufactured by R&D Systems
Sourced in United States
Recombinant TGF-β1 protein is a laboratory product produced using recombinant DNA technology. It is a member of the transforming growth factor beta (TGF-β) family of cytokines. TGF-β1 is a multifunctional protein that regulates a variety of cellular processes, including cell growth, differentiation, and immune function.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Sb431542, by Abcam (1 mentions)
Af 246 na, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Merck Group (1 mentions)
Anti tgf β1 antibody, by R&D Systems (1 mentions)
Real time pcr master mix, by Roche (1 mentions)
2 protocols using recombinant tgf β1 protein
1
Treg Modulation of GC Cell Lines
2
Autonomous Synthesis and Characterization of IMB-S7
Check if the same lab product or an alternative is used in the 5 most similar protocols
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IMB-S7 was synthesized autonomously in our laboratory, and its purity was more than 98% (HPLC). The pcDNA3.1-KLF5 plasmid was purchased from Youbio (Changsha, China), and pCMV3-Myc-ITGAV was purchased from Sino Biological Inc. (Beijing, China); pcDNA3.1-SP1 was kindly provided by Dr Li Wang (Institute of Medicinal Biotechnology, China). Real-time PCR master mix was purchased from Roche (Indianapolis, USA).
Integrin αv antibody was purchased from BD Biosciences (Becton, USA). Antibodies against Sp1, phosphor-Smad2/3, Smad2, and GAPDH were purchased from Cell Signaling Technology (Danvers, USA). Anti-α-SMA and anti-COL1A1 antibodies were obtained from Abnova (Taipei, Chinese Taiwan). Anti-TGF-β1 antibody and recombinant TGF-β1 protein were acquired from R&D Systems (Minneapolis, USA).
Integrin αv antibody was purchased from BD Biosciences (Becton, USA). Antibodies against Sp1, phosphor-Smad2/3, Smad2, and GAPDH were purchased from Cell Signaling Technology (Danvers, USA). Anti-α-SMA and anti-COL1A1 antibodies were obtained from Abnova (Taipei, Chinese Taiwan). Anti-TGF-β1 antibody and recombinant TGF-β1 protein were acquired from R&D Systems (Minneapolis, USA).
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