Wings of 5-day-old males raised at 20°C were mounted as in (20 (link)) and imaged under a Leica Macroscope equipped with a Manta G-609B/C camera (GigE camera with Sony ICX694, Allied Vision, Exton, PA) driven by nVision software (Impuls Imaging GmbH, Türkheim) using a diffuse back lighting table (DBL-2020-WT, MBJ Imaging, Hamburg) for illumination. Images of wings that were broken, damaged, or folded were excluded from the analysis. The number of images analyzed per species was as follows: D. eugracilis, 48; D. biarmipes, 43; D. suzukii, 54; D. prostipennis, 40; D. elegans, 50; D. fuyamai, 50; D. kurseongensis, 52; D. prolongata, 47; D. rhopaloa, 50; D. carrolli, 50; and D. ananassae, 42. Each image represents a biological replicate of the experiment.
Macroscope
Manufactured by Leica
Sourced in France
The Leica Macroscope is a versatile optical instrument designed for detailed examination and analysis of small objects. It provides high-quality magnification and imaging capabilities for a wide range of applications, including industrial, scientific, and research settings. The Macroscope's core function is to enable users to observe and inspect minute details with exceptional clarity and precision.
Automatically generated - may contain errors
6 protocols using macroscope
1
Comparative Wing Morphology Imaging
2
Staining and Imaging Microgel Slices
3
Gut Morphology Assay Protocol
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The mesentery of the gut was carefully removed in PBS with tweezers because it prevented a clear assessment of morphological changes induced by culture; guts were laid flat by reducing the depth of PBS and photographed with a macroscope (Leica) in transmitted light. Small weights were obtained by cutting stainless steel pins (Euronexia, diameter 100–250 µm) to the required size and were weighed with a precision balance (Sartorius). These masses were attached to the distal most part of the hindgut; a full-length (4 cm) stainless steel pin was inserted in the stomach. The gut + pins were next transferred to a 15 mL plastic test tube filled with DMEM GlutaMAXTM-I (Thermoscientific, with 4.5 g/L D-glucose and sodium pyruvate) supplemented with 1% penicillin-streptomycin; the upper full-length pin rested on two wedges cut out at the tube opening; the lower pin (weight) pulled on the gut by gravity. Each gut was placed in an individual test tube; up to 8 guts were incubated simultaneously in a humidified incubator (Thermos) at 37.5 °C in a 5%CO2/95% air atmosphere. After 48 h, the guts were placed in individual Petri dishes in PBS at room-temperature, let to relax for 30 min and photographed. Guts were then further used to determine dry mass, histology or for dissociation assays.
4
Quantifying Drosophila Egg Laying and Adult Progeny
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Virgin female flies were crossed to w1118 males with a ratio of two males per female and putted on agar plates at 25°C. The number of laid eggs was quantified 3 days after the initial cross. Photos of the laid eggs were taken using a Leica macroscope. For quantification of adult progenies, at least three tubes containing five females of each genotype of interest crossed to three w1118 males, were placed at 25°C, and emerged adult progenies (F1 generation) were counted.
5
Fingermark Analysis by Microscopic Techniques
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Fingermarks were recorded photographically and examined with a Leica macroscope as well as being assigned a grade on a 0-4 scale. A selected sample of fingermarks was examined by SEM and EDX. In addition a number of fingermark films were examined by confocal microscope (Olympus LEXT OLS4000) and opto-digital microscope (Olympus DSX100).
In the case of aluminium, copper and brass, attempts were made to remove the oxide layer for examination. Aluminium oxide can be removed by amalgamation of the aluminium using mercury chloride solution. Copper forms a thick brittle oxide film that can fragment due to the different thermal expansion coefficients of oxide and metal and the oxide film could often be removed by applying a piece of forensic lifting tape.
In the case of aluminium, copper and brass, attempts were made to remove the oxide layer for examination. Aluminium oxide can be removed by amalgamation of the aluminium using mercury chloride solution. Copper forms a thick brittle oxide film that can fragment due to the different thermal expansion coefficients of oxide and metal and the oxide film could often be removed by applying a piece of forensic lifting tape.
6
Intravital Imaging of Microvessel Thrombosis
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After a partial dura-sparing craniotomy, rats were subjected to a 60-minute tMCAO (n=10). Intravital fluorescence microscopy (Macroscope, Leica, France) was used to visualize circulating blood cells (after intravenous injection of rhodamine 6G) and fibrinogen (fluorescein isothiocyanate [FITC] antifibrinogen polyclonal antibody, Dako, France) during MCAO and after recanalization. Microvessel thrombosis was defined as direct visualization of platelet, leukocyte, and fibrinogen aggregates.
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