Anti arg1 sc 271430

Immunoblotting was performed as previously described [48 (link)] using the following primary antibodies: anti-iNOS (ab15323, Abcam), anti-CD16 (ab203883), anti-IFNγ (500-P119, PeproTech, Rocky Hill, NJ, USA), anti-Arg1 (sc-271430, Santa Cruz), anti-CD206 (ab64693, Abcam), anti-IL-4 (ab11524, Abcam), anti-STAT1 (ab3987, Abcam), anti-p-STAT1 (ab30645, Abcam), anti-STAT6 (ab32520, Abcam), anti-p-STAT6 (ab28829, Abcam), anti-SOCS3 (ab16030, Abcam), and the HRP-conjugated secondary antibody (1:5000). The plots were visualized by ECL Plus (Thermo).

Paraformaldehyde was used to fix BMDMs on glass slides, after which they were blocked using goat serum prior to probing overnight at 4°C with anti-Arg-1 (16001-1-AP; 1:200; Proteintech Group, Wuhan, China) or anti-iNOS (610431; 1:200; BD Biosciences). Samples were then stained using secondary antibodies labelled with Cy3 or AF488 (1:1000; Thermo Fisher Scientific), followed by DAPI staining and mounting. Samples were assessed via fluorescence microscopy (Nikon, Tokyo, Japan), with all samples being assessed with the same settings.
Deparaffinization and antigen retrieval were employed to prepare murine paraffin-embedded renal tissue sections, which were then treated with goat serum as a blocking reagent prior to overnight incubation at 4°C with anti-Arg-1 (sc-271430; 1:200; Santa Cruz Biotechnology, TX, USA) or anti-iNOS (BA0362, 1:200, Boster). Samples were then probed with secondary antibodies conjugated to FITC or Cy3 (1:1000; Thermo Fisher Scientific), followed by fluorescence microscopic evaluation as above.