MRI was conducted using three different systems as the study overlapped with the decommissioning of an older MRI unit (Siemens 1.5 Tesla Symphony), installation of an upgraded system (Siemens 3.0 Tesla Skyra), and use of a “bridge” unit (Siemens 1.5 Tesla Symphony equipped with older software). Sequences obtained included T1‐weighted fast spin echo (FSE), T2‐weighted FSE, T1‐ and T2‐weighted fluid attenuated inversion recovery, gradient echo (T2*), and susceptibility‐weighted imaging. T1‐weighted images were also obtained after administration of Gadoversetamide (0.1 mmol/kg, Optimark, Mallinckrodt Inc., St. Louis, MO). For the initial MRI studies, a T1‐weighted, 3D volumetric sequence was also obtained (MP‐RAGE) for use with a veterinary neuronavigation system (BrainSight, Rogue Research, Montreal, Canada). Bite plates affixed with MRI‐compatible fiducial markers were also utilized in this initial MRI study as a prelude to neuronavigation and were created using Hydroplastic impression material (Tak Systems, Wareham, MA).
Bomba H.N., Sheets K.T., Valdivia A., Khagi S., Ruterbories L., Mariani C.L., Borst L.B., Tokarz D.A, & Hingtgen S.D. (2020). Personalized‐induced neural stem cell therapy: Generation, transplant, and safety in a large animal model. Bioengineering & Translational Medicine, 6(1), e10171.
+ Open protocol