HA sodium salt (molecular weight = 10–12 kDa) was purchased from Lifecore Biomedical (MN, USA). Methoxy poly(ethylene glycol) amine (PEG‐NH2, molecular weight = 5 kDa) was obtained from Laysan Bio Inc. (AL, USA). Cy5.5‐DBCO and Ac4ManNAz were purchased from Click Chemistry Tools (AZ, USA). Thiazolyl blue tetrazolium bromide (MTT), DBCO‐amine and dimethylformamide (DMF) were obtained from Sigma‐Aldrich Co. (MO, USA). Sodium cyanoborohydride (NaBH3CN) was purchased from TCI (Tokyo, Japan). Poly(vinylidene fluoride) membrane was obtained from Millipore (MA, USA). Cy5.5 NHS ester dye was purchased from GE Healthcare (IL, USA). Foetal bovine serum (FBS), RPMI 1640, Dulbecco's phosphate‐buffered saline (DPBS), antibiotic‐antimycotic (AA) solution (100×) and trypsin‐trypsin‐ethylene diamine tetraacetic acid were purchased from WelGENE (Gyeongsan, Korea). NIH/3T3, CT26, RAW264.7, MDA‐MB‐231 and HCT‐116 were provided by the Korean Cell Line Bank (Seoul, Korea). Water was purified using an AquaMax‐Ultra water purification system from Younglin Co. (Anyang, Korea). All other chemicals were of analytical grade and used without further purification. Information on the antibodies used in the experiments is listed in Table S1 .
Cy5.5 nhs ester dye
Manufactured by GE Healthcare
Sourced in United States
Cy5.5 NHS ester dye is a fluorescent labeling reagent used for the covalent attachment of dyes to biomolecules. It is designed for labeling proteins, peptides, and other compounds containing primary amine groups.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Ha sodium salt, by Lifecore Biomedical (1 mentions)
Hct116, by Korean Cell Line Bank (1 mentions)
Mda mb 231, by Korean Cell Line Bank (1 mentions)
Ac4mannaz, by Vector Laboratories (1 mentions)
Cy5.5 dbco, by Vector Laboratories (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Welgene (1 mentions)
Nih3t3, by Korean Cell Line Bank (1 mentions)
Raw 264, by Korean Cell Line Bank (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
Aquamax ultra water purification system, by Younglin (1 mentions)
2 protocols using cy5.5 nhs ester dye
1
Glycan-Mediated Tumor Cell Targeting
2
Biodistribution of Cy5.5-labeled VNVs
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The animals were divided into the following three experimental groups: PBS, IM, and IN. The PBS group received 50 µL of PBS via the IN route and 100 µL of PBS via the IM route. The IM group received 100 µL of a solution containing 50 µg of VNVs and 250 µg of alum via the IM route. The IN group received 50 µL of a solution consisting of 50 µg of VNVs and 2.5 µg of CT via the IN route. Prior to administration, the VNVs were labelled with cy5.5 NHS ester dye (GE, PA15601) and any unbound dyes were removed through two rounds of ultracentrifugation. The mice were euthanized at 0.2, 4, and 24 h after administration, and vital organs (brain, heart, lung, liver, spleen, and kidney) were isolated for fluorescence imaging to detect Cy5.5 signals originating from the VNVs.
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