The antibodies for immunoprecipitation were anti-LSD1 (ab17721, Abcam, Cambridge, UK), anti-GFI1B (sc-28356X, Santa Cruz Biotech, Dallas, TX, USA), anti-RUNX1 (ab23980, Abcam), anti-H3K27ac (39133, Active Motif, Carlsbad, CA, USA), anti-CoREST (ab32631, Abcam), normal rabbit IgG (sc-2027, Santa Cruz), and normal mouse IgG (sc-2025, Santa Cruz). The primary antibodies for western blotting were anti-LSD1 (C69G12, Millipore, Billerica, MA, USA), anti-CoREST (ab32631, Abcam), anti-HDAC1 (ab7028, Abcam), anti-HDAC2 (ab7029, Abcam), anti-GFI1B (sc-28356X or sc-22795, Santa Cruz), anti-RUNX1 (ab23980, Abcam, or sc-365644, Santa Cruz), anti-ERG (ab133264, Abcam), and anti-ACTIN (sc-1616, Santa Cruz). The secondary antibodies were anti-rabbit IgG (NA934V, GE Healthcare, Little Chalfont, UK), anti-mouse IgG (NA931V, GE Healthcare), and anti-goat IgG (sc-2020, Santa Cruz) conjugated with horseradish peroxidase.
Yamamoto R., Kawahara M., Ito S., Satoh J., Tatsumi G., Hishizawa M., Suzuki T, & Andoh A. (2018). Selective dissociation between LSD1 and GFI1B by a LSD1 inhibitor NCD38 induces the activation of ERG super-enhancer in erythroleukemia cells. Oncotarget, 9(30), 21007-21021.
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