Upon arrival, NS-agar cultures were incubated for up to two days at 37 °C and 5% CO2. On both days cultures were screened for presence of meningococcus-like colonies (grey, round and smooth colonies with convex shape). When found, 1–3 colonies were re-plated on Columbia Blood agar (CBA, bioTRADING Benelux B.V., Mijdrecht, the Netherlands) and tested for species identification using Matrix-assisted Laser Desorption/Ionization Time-of-Flight mass spectronomy (MALDI-ToF, Bruker Daltonik GmbH, Bremen, Germany). Separately for oropharyngeal and saliva samples, a single isolate with a score ≥ 2.0 for Neisseria meningitidis (database BDAL V8.0.0.0 + SR1.0.0.0, Bruker Daltonik) was stored at − 70 °C in Brain Heart Infusion (BHI, Oxoid) supplemented with 0.5% Yeast Extract (YE, Oxoid) and 10% glycerol. NS-agar cultures displaying any microbial growth were harvested into 2 ml of Todd–Hewitt Broth (Oxoid) supplemented with 0.5% YE and 10% glycerol. These harvests were considered to be culture-enriched for meningococci, and 0.7 ml of it stored at − 70 °C.
Bdal v8 0 0 0 sr1
Manufactured by Bruker
Sourced in Germany
The BDAL V8.0.0.0 + SR1.0.0.0 is a lab equipment product from Bruker. It serves as a core function for laboratory applications.
Automatically generated - may contain errors
2 protocols using bdal v8 0 0 0 sr1
1
Meningococcal Isolation and Identification
2
Isolation and Identification of Neisseria meningitidis
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Upon arrival, NS-agar cultures were incubated for up to two days at 37°C and 5% CO2. On both days cultures were screened for presence of meningococcus-like colonies (grey, round and smooth colonies with convex shape). When found, 1-3 colonies were re-plated on Columbia Blood agar (CBA, bioTRADING Benelux B.V., Mijdrecht, the Netherlands) and tested for species identification using Matrix-assisted Laser Desorption/Ionization Time-of-Flight mass spectronomy (MALDI-ToF; Bruker Daltonik GmbH, Bremen, Germany).
Separately for OP and saliva samples, a single isolate with a score ≥2.0 for Neisseria meningitidis (database BDAL V8.0.0.0+SR1.0.0.0, Bruker Daltonik) was stored at -70°C in Brain Heart Infusion (BHI, Oxoid) supplemented with 0.5% Yeast Extract (YE; Oxoid) and 10% glycerol. NS-agar cultures displaying any microbial growth were harvested into 2 ml of Todd-Hewitt Broth (Oxoid) supplemented with 0.5% YE and 10% glycerol. These harvests were considered to be culture-enriched for meningococci, and 0.7 ml of it stored at -70°C.
Separately for OP and saliva samples, a single isolate with a score ≥2.0 for Neisseria meningitidis (database BDAL V8.0.0.0+SR1.0.0.0, Bruker Daltonik) was stored at -70°C in Brain Heart Infusion (BHI, Oxoid) supplemented with 0.5% Yeast Extract (YE; Oxoid) and 10% glycerol. NS-agar cultures displaying any microbial growth were harvested into 2 ml of Todd-Hewitt Broth (Oxoid) supplemented with 0.5% YE and 10% glycerol. These harvests were considered to be culture-enriched for meningococci, and 0.7 ml of it stored at -70°C.
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