Abf23

Manufactured by Merck Group

The ABF23 is a laboratory equipment product manufactured by Merck Group. It is a high-precision instrument designed for conducting various analytical and experimental procedures in a controlled laboratory environment. The core function of the ABF23 is to provide accurate and reliable measurements, data collection, and analysis capabilities to support scientific research and product development activities.

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Lab products found in correlation

Duolink pla technology, by Merck Group (2 mentions)

2 protocols using abf23

Protein Interaction Visualization in Cell Lines

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HBEC3-KT, MDA-MB231, and HeLa cells were seeded onto glass coverslips, transfected as previously described and processed as described by the manufacturer’s protocol (Duolink PLA Technology, Sigma-Aldrich) using NLRP3 2 μg/ml (ABF23; Millipore) and ATM 2 μg/ml (2C1; Abcam) antibodies. DAPI was used to stain nuclei. Quantification was carried out using the macro published by Poulard et al (2019) (link).

Bodnar-Wachtel M., Huber A.L., Gorry J., Hacot S., Burlet D., Gérossier L., Guey B., Goutagny N., Bartosch B., Ballot E., Lecuelle J., Truntzer C., Ghiringhelli F., Py B.F., Couté Y., Ballesta A., Lantuejoul S., Hall J., Tissier A, & Petrilli V. (2023). Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress. Life Science Alliance, 6(4), e202201494.

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Proximity Ligation Assay for NLRP3 and ATM

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HBECT3-KT and HeLa cells were seeded onto glass coverslips and processed as described by the manufacturer's protocol (Duolink® PLA Technology, Sigma). Antibodies used NLRP3 1/500 (ABF23, Millipore), ATM 1/500 (2C1, Abcam). Quantification was carried out using the macro published by Poulard et al 42 (link) .

Bodnar‐Wachtel M., Huber A., Gorry J., Hacot S., Gérossier L., Guey B., Goutagny N., Bartosch B., Ballot E., Ghiringhelli F., Py B.F., Couté Y., Ballesta A., Lantuéjoul S., Hall J., & Pétrilli V. (2020). NLRP3 controls ATM activation in response to DNA damage.

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